Changes of metal-induced toxicity by H2O2/NO modulators in Scenedesmus quadricauda (Chlorophyceae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A00210886" target="_blank" >RIV/62156489:43210/13:00210886 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11356-013-1541-0" target="_blank" >http://dx.doi.org/10.1007/s11356-013-1541-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11356-013-1541-0" target="_blank" >10.1007/s11356-013-1541-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Changes of metal-induced toxicity by H2O2/NO modulators in Scenedesmus quadricauda (Chlorophyceae)

Popis výsledku v původním jazyce

Effect of nitric oxide donor (sodium nitroprusside, SNP, 500 mu M) or hydrogen peroxide scavenger (dithiothreitol, DTT, 500 mu M) on cadmium (Cd) or copper (Cu) uptake (150 mu M solutions) and toxicity using Scenedesmus quadricauda was studied. Combinedtreatments (Cd or Cu + DTT or SNP) usually ameliorated metal-induced toxicity at the level of pigments, proteins, and mineral nutrients in comparison with respective metal alone. Viability tests (MTT and TTC) showed the lowest values preferentially in Cutreatments, indicating higher toxicity in comparison with Cd. Cd showed low impact on amino acids while strong Cu-induced depletion was mitigated by DTT and SNP. Amount of ROS and NO showed the most pronounced responses in SNP variants being rather reciprocal than parallel and regulated ascorbate peroxidase activity. Blot gel analyses of hsp70 protein did not reveal extensive changes after given exposure period. Phenols were elevated by DTT alone while all Cu treatments revealed depleti

Název v anglickém jazyce

Changes of metal-induced toxicity by H2O2/NO modulators in Scenedesmus quadricauda (Chlorophyceae)

Popis výsledku anglicky

Effect of nitric oxide donor (sodium nitroprusside, SNP, 500 mu M) or hydrogen peroxide scavenger (dithiothreitol, DTT, 500 mu M) on cadmium (Cd) or copper (Cu) uptake (150 mu M solutions) and toxicity using Scenedesmus quadricauda was studied. Combinedtreatments (Cd or Cu + DTT or SNP) usually ameliorated metal-induced toxicity at the level of pigments, proteins, and mineral nutrients in comparison with respective metal alone. Viability tests (MTT and TTC) showed the lowest values preferentially in Cutreatments, indicating higher toxicity in comparison with Cd. Cd showed low impact on amino acids while strong Cu-induced depletion was mitigated by DTT and SNP. Amount of ROS and NO showed the most pronounced responses in SNP variants being rather reciprocal than parallel and regulated ascorbate peroxidase activity. Blot gel analyses of hsp70 protein did not reveal extensive changes after given exposure period. Phenols were elevated by DTT alone while all Cu treatments revealed depleti

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Science and Pollution Research

ISSN

0944-1344

e-ISSN

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Svazek periodika

20

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

5502-5511

Kód UT WoS článku

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EID výsledku v databázi Scopus

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