Shoot regeneration from various explants of horse chestnut (Aesculus hippocastanum L.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A00212775" target="_blank" >RIV/62156489:43210/13:00212775 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027073:_____/13:#0001614

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.scienta.2013.06.030" target="_blank" >http://dx.doi.org/10.1016/j.scienta.2013.06.030</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.scienta.2013.06.030" target="_blank" >10.1016/j.scienta.2013.06.030</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Shoot regeneration from various explants of horse chestnut (Aesculus hippocastanum L.)

Popis výsledku v původním jazyce

A protocol is described for the adventitious shoot bud regeneration from leaf, stem and root explants in A. hippocastanum. Different medium (Heller, WPM, Rugini and DKW) and growth regulators [N6-benzyladenine (BA), thidiazuron (TDZ), and naphthaleneacetic acid (NAA)] at a range of various concentrations have been investigated for organogenesis. Nutrient content in the culture media had a significant effect on regeneration of new shoots in Aesculus. The highest number and length of shoots was achieved on Rugini and WPM medium with moderate nutrient content. Adventitious shoot formation was observed in all types of explants. Regeneration frequency and shoot number depended on explant type and growth regulators. The highest regeneration (100%) and shootbud number (9.68) were recorded in stem explants on medium with 2 mg l--1 BA + 0.1 mg l--1 NAA. Generally, TDZ has lower effect on shoot regeneration in stem and leaf explants compared to BA. The additions of NAA to the culture medium imp

Název v anglickém jazyce

Shoot regeneration from various explants of horse chestnut (Aesculus hippocastanum L.)

Popis výsledku anglicky

A protocol is described for the adventitious shoot bud regeneration from leaf, stem and root explants in A. hippocastanum. Different medium (Heller, WPM, Rugini and DKW) and growth regulators [N6-benzyladenine (BA), thidiazuron (TDZ), and naphthaleneacetic acid (NAA)] at a range of various concentrations have been investigated for organogenesis. Nutrient content in the culture media had a significant effect on regeneration of new shoots in Aesculus. The highest number and length of shoots was achieved on Rugini and WPM medium with moderate nutrient content. Adventitious shoot formation was observed in all types of explants. Regeneration frequency and shoot number depended on explant type and growth regulators. The highest regeneration (100%) and shootbud number (9.68) were recorded in stem explants on medium with 2 mg l--1 BA + 0.1 mg l--1 NAA. Generally, TDZ has lower effect on shoot regeneration in stem and leaf explants compared to BA. The additions of NAA to the culture medium imp

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/QH81101" target="_blank" >QH81101: Preventivní ochrana nových výsadeb Aesculus hippocastanum s využitím klonu Mertelik06 rezistentního ke Cameraria ohridella</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientia Horticulturae

ISSN

0304-4238

e-ISSN

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Svazek periodika

161

Číslo periodika v rámci svazku

September 24

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

223-227

Kód UT WoS článku

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EID výsledku v databázi Scopus

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