Study of interaction between metallothionein and CdTe quantum dots

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A00213473" target="_blank" >RIV/62156489:43210/13:00213473 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/13:43895529 RIV/00216305:26620/13:PU126542

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10337-013-2418-6" target="_blank" >http://dx.doi.org/10.1007/s10337-013-2418-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10337-013-2418-6" target="_blank" >10.1007/s10337-013-2418-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Study of interaction between metallothionein and CdTe quantum dots

Popis výsledku v původním jazyce

Quantum dots (QDs) belong to a new class of fluorescent agent for biochemical, medicinal or other purposes. However, QDs based on cadmium or other metals can be risky for an organism. As one of the mechanism how to detoxify cadmium-based QDs expression of metallothioneins (MT) can be considered. Due to high affinity of metallothionein to cadmium(II) ions, we attempted to develop an approach for studying of possible interaction with QDs. We prepared QDs with CdTe core and studied the interaction with MT,which we isolated from livers of Cd-administered rabbits. To study the interaction, we used the mixture of both components MT (3.6 uM): CdTe QDs (0, 0.34, 0.68, 1.02, 1.36, 1.7, 2.04 and 2.47 uM). The mixtures were studied by spectrophotometry within the range from 200 to 750 nm with detected maxima at 260 and 505 nm. Same mixtures were also analysed by differential pulse voltammetry Brdicka reaction, which supported data from spectrophotometry. Subsequently, we used fast protein liquid

Název v anglickém jazyce

Study of interaction between metallothionein and CdTe quantum dots

Popis výsledku anglicky

Quantum dots (QDs) belong to a new class of fluorescent agent for biochemical, medicinal or other purposes. However, QDs based on cadmium or other metals can be risky for an organism. As one of the mechanism how to detoxify cadmium-based QDs expression of metallothioneins (MT) can be considered. Due to high affinity of metallothionein to cadmium(II) ions, we attempted to develop an approach for studying of possible interaction with QDs. We prepared QDs with CdTe core and studied the interaction with MT,which we isolated from livers of Cd-administered rabbits. To study the interaction, we used the mixture of both components MT (3.6 uM): CdTe QDs (0, 0.34, 0.68, 1.02, 1.36, 1.7, 2.04 and 2.47 uM). The mixtures were studied by spectrophotometry within the range from 200 to 750 nm with detected maxima at 260 and 505 nm. Same mixtures were also analysed by differential pulse voltammetry Brdicka reaction, which supported data from spectrophotometry. Subsequently, we used fast protein liquid

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chromatographia

ISSN

0009-5893

e-ISSN

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Svazek periodika

76

Číslo periodika v rámci svazku

7-8

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

345-353

Kód UT WoS článku

316819600007

EID výsledku v databázi Scopus

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