Ultrastructural energetic model of porcine oocytes with different meiotic competence

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A43908823" target="_blank" >RIV/62156489:43210/13:43908823 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2013/mnet_2013_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2013/mnet_2013_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Ultrastructural energetic model of porcine oocytes with different meiotic competence

Popis výsledku v původním jazyce

The principal objective of the study was to characterize energetic components of matured porcine oocytes with different meiotic competence. Lipid droplets (LDs), activity of mitochondria and adenosine triphosphate (ATP) content represent the constituentsimportant for cytoplasmic maturation of mammalian oocytes. This research provides detection of LDs, ATP content and rearrangement of mitochondria in matured porcine oocytes with different meiotic competence. In porcine oocytes mitochondria surround LDsforming so-called metabolic units. Meiotic competence of porcine oocytes is directly influenced by the size of follicle from which oocytes are derived. The meiotically higher competent (MHC) and less competent (MLC) oocytes were isolated separately frommedium (6-9 mm) and small follicles (<5 mm) respectively by aspiration and cutting of ovarian cortex. LDs were stained by molecular probe Nile red. To determine localization of the lipid droplets protein, oocytes were labeled with an anti

Název v anglickém jazyce

Ultrastructural energetic model of porcine oocytes with different meiotic competence

Popis výsledku anglicky

The principal objective of the study was to characterize energetic components of matured porcine oocytes with different meiotic competence. Lipid droplets (LDs), activity of mitochondria and adenosine triphosphate (ATP) content represent the constituentsimportant for cytoplasmic maturation of mammalian oocytes. This research provides detection of LDs, ATP content and rearrangement of mitochondria in matured porcine oocytes with different meiotic competence. In porcine oocytes mitochondria surround LDsforming so-called metabolic units. Meiotic competence of porcine oocytes is directly influenced by the size of follicle from which oocytes are derived. The meiotically higher competent (MHC) and less competent (MLC) oocytes were isolated separately frommedium (6-9 mm) and small follicles (<5 mm) respectively by aspiration and cutting of ovarian cortex. LDs were stained by molecular probe Nile red. To determine localization of the lipid droplets protein, oocytes were labeled with an anti

Druh

D - Stať ve sborníku

CEP obor

GI - Šlechtění a plemenářství hospodářských zvířat

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2013: Proceedings of International PhD Students Conference

ISBN

978-80-7375-908-7

ISSN

—

e-ISSN

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Počet stran výsledku

9

Strana od-do

238-246

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

20. 11. 2013

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000366464800041