Porcine ubiquitin-like 5 (UBL5) gene: genomic organization, polymorphisms, mRNA cloning, splicing variants and association study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A00215776" target="_blank" >RIV/62156489:43210/14:00215776 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985904:_____/14:00427358

Výsledek na webu

<a href="http://link.springer.com/article/10.1007%2Fs11033-014-3089-6" target="_blank" >http://link.springer.com/article/10.1007%2Fs11033-014-3089-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11033-014-3089-6" target="_blank" >10.1007/s11033-014-3089-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Porcine ubiquitin-like 5 (UBL5) gene: genomic organization, polymorphisms, mRNA cloning, splicing variants and association study

Popis výsledku v původním jazyce

Ubiquitin-like 5 (UBL5), which is supposed to be involved in regulation of feed intake, energy metabolism, obesity and type 2 diabetes, is located at position 62.1 cM on the pig chromosome 2 region harbouring quantitative trait loci for carcass and meatquality. The 4,354 bp genomic sequence (FR798948) of the porcine gene encompassing the promoter and entire gene was cloned by polymerase chain reaction. Comparative sequencing revealed 13 polymorphisms in noncoding regions. Synthesis of full-length cDNAsequences using rapid amplification of 50 and 30 ends showed three splice variants. Variants 1 and 2 differ in transcription length for the untranslated part of exon 1 with deduced protein of 73 amino acid (aa) residues and 100 % identities between human, mouse and other species. Variant 3, with 4 bp deletion at the 30 end of exon 2, encodes a truncated protein with 28 aa residues. In a Wild boar9Meishan F2 population (n = 334) with 47 recorded traits, loci FR798948:g.2788G[A and FR79894

Název v anglickém jazyce

Porcine ubiquitin-like 5 (UBL5) gene: genomic organization, polymorphisms, mRNA cloning, splicing variants and association study

Popis výsledku anglicky

Ubiquitin-like 5 (UBL5), which is supposed to be involved in regulation of feed intake, energy metabolism, obesity and type 2 diabetes, is located at position 62.1 cM on the pig chromosome 2 region harbouring quantitative trait loci for carcass and meatquality. The 4,354 bp genomic sequence (FR798948) of the porcine gene encompassing the promoter and entire gene was cloned by polymerase chain reaction. Comparative sequencing revealed 13 polymorphisms in noncoding regions. Synthesis of full-length cDNAsequences using rapid amplification of 50 and 30 ends showed three splice variants. Variants 1 and 2 differ in transcription length for the untranslated part of exon 1 with deduced protein of 73 amino acid (aa) residues and 100 % identities between human, mouse and other species. Variant 3, with 4 bp deletion at the 30 end of exon 2, encodes a truncated protein with 28 aa residues. In a Wild boar9Meishan F2 population (n = 334) with 47 recorded traits, loci FR798948:g.2788G[A and FR79894

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GI - Šlechtění a plemenářství hospodářských zvířat

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Biology Reports

ISSN

0301-4851

e-ISSN

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Svazek periodika

41

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

2353-2362

Kód UT WoS článku

333707100051

EID výsledku v databázi Scopus

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