3D printed chip for electrochemical detection of influenza virus labeled with CdS quantum dots
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A00217377" target="_blank" >RIV/62156489:43210/14:00217377 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216305:26620/14:PU107958
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.bios.2013.10.031" target="_blank" >http://dx.doi.org/10.1016/j.bios.2013.10.031</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bios.2013.10.031" target="_blank" >10.1016/j.bios.2013.10.031</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
3D printed chip for electrochemical detection of influenza virus labeled with CdS quantum dots
Popis výsledku v původním jazyce
In this study,we report a new three-dimensional (3D), bead-based microfluidic chip developed for rapid, sensitive and specific detection of influenza hemagglutinin. The principle of microfluidic chip is based on implementation of two-step procedure thatincludes isolation based on paramagnetic beads and electrochemical detection. As a platform for isolation process, streptavidin-modified MPs,which were conjugated via biotinylated glycan (through streptavidin--biotin affinity) followed by linkage of hemagglutinin to glycan, were used. Vaccine hemagglutinin (HAvaxi) was labeled with CdS quantum dots (QDs) at first. Detection of the isolation product by voltammetry was the end point of the procedure. The suggested and developed method can be used also fordetection of other specific substances that are important for control, diagnosis or therapy of infectious diseases.
Název v anglickém jazyce
3D printed chip for electrochemical detection of influenza virus labeled with CdS quantum dots
Popis výsledku anglicky
In this study,we report a new three-dimensional (3D), bead-based microfluidic chip developed for rapid, sensitive and specific detection of influenza hemagglutinin. The principle of microfluidic chip is based on implementation of two-step procedure thatincludes isolation based on paramagnetic beads and electrochemical detection. As a platform for isolation process, streptavidin-modified MPs,which were conjugated via biotinylated glycan (through streptavidin--biotin affinity) followed by linkage of hemagglutinin to glycan, were used. Vaccine hemagglutinin (HAvaxi) was labeled with CdS quantum dots (QDs) at first. Detection of the isolation product by voltammetry was the end point of the procedure. The suggested and developed method can be used also fordetection of other specific substances that are important for control, diagnosis or therapy of infectious diseases.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>
Návaznosti
O - Projekt operacniho programu
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Biosensors and Bioelectronics
ISSN
0956-5663
e-ISSN
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Svazek periodika
54
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
7
Strana od-do
421-427
Kód UT WoS článku
333071500064
EID výsledku v databázi Scopus
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