Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A00230473" target="_blank" >RIV/62156489:43210/14:00230473 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/14:PU112580

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10337-014-2737-2" target="_blank" >http://dx.doi.org/10.1007/s10337-014-2737-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10337-014-2737-2" target="_blank" >10.1007/s10337-014-2737-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza

Popis výsledku v původním jazyce

Considerable efforts have been devoted to the development of rapid and sensitive methods allowing the detection of viral nucleic acid. We herein describe an assay for identification of a specific influenza sequence. The suggested method was based on isolation using paramagnetic particles coupled with electrochemical detection of isolated product. Peptide nucleic acid (PNA) was used as a probe for hybridization and identification of the influenza-derived specific sequence. The use of PNA can show numerous benefits: PNA probe is not degradable by enzymes and the duplex of PNA with RNA/DNA is more thermostable and more resistant to pH changes than DNA/DNA or RNA/RNA duplexes. This PNA probe assay can be applied as a magnetically guidable tool for detection of DNA/RNA samples under different conditions.

Název v anglickém jazyce

Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza

Popis výsledku anglicky

Considerable efforts have been devoted to the development of rapid and sensitive methods allowing the detection of viral nucleic acid. We herein describe an assay for identification of a specific influenza sequence. The suggested method was based on isolation using paramagnetic particles coupled with electrochemical detection of isolated product. Peptide nucleic acid (PNA) was used as a probe for hybridization and identification of the influenza-derived specific sequence. The use of PNA can show numerous benefits: PNA probe is not degradable by enzymes and the duplex of PNA with RNA/DNA is more thermostable and more resistant to pH changes than DNA/DNA or RNA/RNA duplexes. This PNA probe assay can be applied as a magnetically guidable tool for detection of DNA/RNA samples under different conditions.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

O - Projekt operacniho programu

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chromatographia

ISSN

0009-5893

e-ISSN

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Svazek periodika

77

Číslo periodika v rámci svazku

21-22

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

1425-1432

Kód UT WoS článku

345004800003

EID výsledku v databázi Scopus

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