Hyphenated Solid Phase Extraction/Supercritical Fluid Extraction Methods for Extraction of Phenolic Compounds from Algae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A43908664" target="_blank" >RIV/62156489:43210/14:43908664 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Hyphenated Solid Phase Extraction/Supercritical Fluid Extraction Methods for Extraction of Phenolic Compounds from Algae

Popis výsledku v původním jazyce

Hyphenated SPE/SFE method was developed for the extraction of phenolic compounds from plant matrices (6 algae species). 20 ml of liquid solvent was injected to the SPE cartridge and extracted at following conditions: 40 MPa, 80 degrees C, restrictor 10.5cm x 50 mu m i.d. for 60 min into 20 ml of MeOH as a trapping solvent. Two different methods were developed for different SPE sorbents (OASIS MCX 3cc, Waters and ADVANTA Spe-ed, Applied Separations) to prove that the principles work at different conditions. Each sorbent needs different modifier: 400 mu L of 2M HAc in situ + 50 % MeOH / 5 % ammonia in ultra-pure water on-line is best for OASIS, ADVANTA needs 50 % MeOH and 5 % ether (v/v) on-line. SPE/SFE method has higher recovery, gives cleaner extracts, enables the use of liquid samples via SPE cartridge and higher precision was achieved (no restrictor plugging). Reversed phase liquid chromatography-electrospray mass spectrometry analysis was used for analyte determination: the chroma

Název v anglickém jazyce

Hyphenated Solid Phase Extraction/Supercritical Fluid Extraction Methods for Extraction of Phenolic Compounds from Algae

Popis výsledku anglicky

Hyphenated SPE/SFE method was developed for the extraction of phenolic compounds from plant matrices (6 algae species). 20 ml of liquid solvent was injected to the SPE cartridge and extracted at following conditions: 40 MPa, 80 degrees C, restrictor 10.5cm x 50 mu m i.d. for 60 min into 20 ml of MeOH as a trapping solvent. Two different methods were developed for different SPE sorbents (OASIS MCX 3cc, Waters and ADVANTA Spe-ed, Applied Separations) to prove that the principles work at different conditions. Each sorbent needs different modifier: 400 mu L of 2M HAc in situ + 50 % MeOH / 5 % ammonia in ultra-pure water on-line is best for OASIS, ADVANTA needs 50 % MeOH and 5 % ether (v/v) on-line. SPE/SFE method has higher recovery, gives cleaner extracts, enables the use of liquid samples via SPE cartridge and higher precision was achieved (no restrictor plugging). Reversed phase liquid chromatography-electrospray mass spectrometry analysis was used for analyte determination: the chroma

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Current Analytical Chemistry

ISSN

1573-4110

e-ISSN

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Svazek periodika

10

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

AE - Spojené arabské emiráty

Počet stran výsledku

13

Strana od-do

86-98

Kód UT WoS článku

000326245500007

EID výsledku v databázi Scopus

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