Fluorescence resonance energy transfer between green fluorescent protein and doxorubicin enabled by DNA nanotechnology
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A43908666" target="_blank" >RIV/62156489:43210/14:43908666 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216305:26620/14:PU126725
Výsledek na webu
<a href="http://dx.doi.org/10.1002/elps.201400166" target="_blank" >http://dx.doi.org/10.1002/elps.201400166</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201400166" target="_blank" >10.1002/elps.201400166</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Fluorescence resonance energy transfer between green fluorescent protein and doxorubicin enabled by DNA nanotechnology
Popis výsledku v původním jazyce
DNA nanotechnology is a rapidly growing research area, where DNA may be used for wide range of applications such as construction of nanodevices serving for large scale of diverse purposes. Likewise a panel of various purified fluorescent proteins is investigated for their ability to emit their typical fluorescence spectra under influence of particular excitation. Hence these proteins may form ideal donor molecules for assembly of fluorescence resonance emission transfer (FRET) constructions. To extend the application possibilities of fluorescent proteins, while using DNA nanotechnology, we developed nanoconstruction comprising green fluorescent protein (GFP) bound onto surface of surface active nanomaghemite and functionalized with gold nanoparticles.We took advantage of natural affinity between gold and thiol moieties, which were modified to bind DNA fragment. Finally we enclosed doxorubicin into fullerene cages. Doxorubicin intercalated in DNA fragment bound on the particles and thu
Název v anglickém jazyce
Fluorescence resonance energy transfer between green fluorescent protein and doxorubicin enabled by DNA nanotechnology
Popis výsledku anglicky
DNA nanotechnology is a rapidly growing research area, where DNA may be used for wide range of applications such as construction of nanodevices serving for large scale of diverse purposes. Likewise a panel of various purified fluorescent proteins is investigated for their ability to emit their typical fluorescence spectra under influence of particular excitation. Hence these proteins may form ideal donor molecules for assembly of fluorescence resonance emission transfer (FRET) constructions. To extend the application possibilities of fluorescent proteins, while using DNA nanotechnology, we developed nanoconstruction comprising green fluorescent protein (GFP) bound onto surface of surface active nanomaghemite and functionalized with gold nanoparticles.We took advantage of natural affinity between gold and thiol moieties, which were modified to bind DNA fragment. Finally we enclosed doxorubicin into fullerene cages. Doxorubicin intercalated in DNA fragment bound on the particles and thu
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Electrophoresis
ISSN
0173-0835
e-ISSN
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Svazek periodika
35
Číslo periodika v rámci svazku
23
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
12
Strana od-do
3290-3301
Kód UT WoS článku
000345972900002
EID výsledku v databázi Scopus
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