Interaction of Strigolactone with polar auxin transport in roots of Arabidopsis thaliana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A43908772" target="_blank" >RIV/62156489:43210/14:43908772 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2014/mnet_2014_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2014/mnet_2014_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Interaction of Strigolactone with polar auxin transport in roots of Arabidopsis thaliana

Popis výsledku v původním jazyce

One of the major regulators of root architecture is the plant hormone auxin. According to recent investigation strigolactone is also involved in this process and is supposed to act by regulation of polar auxin transport mediated by PIN efflux carriers. To choose an optimal concentration for the subsequent study the influence of 12 nM to 50 mu M strigolactone GR24 on Arabidopsis root system was evaluated. 242 ecotypes of Arabidopsis thaliana were analyzed by genome-wide association study (GWAS) under theinfluence of 100 nM of strigolactone. Results of the study are analyzed for a search of candidate genes which participate in strigolactone signaling pathways. From obtained results we have chosen 5 candidate genes (AFB4, F-box, BEN2, F-box associated, MPK) for analysis of their expression levels dependent on strigolactone concentration in Arabidopsis roots and simultaneously auxin efflux (PIN1,2,3,4,7) and influx (AUX1) carriers were analyzed by Real-Time PCR.

Název v anglickém jazyce

Interaction of Strigolactone with polar auxin transport in roots of Arabidopsis thaliana

Popis výsledku anglicky

One of the major regulators of root architecture is the plant hormone auxin. According to recent investigation strigolactone is also involved in this process and is supposed to act by regulation of polar auxin transport mediated by PIN efflux carriers. To choose an optimal concentration for the subsequent study the influence of 12 nM to 50 mu M strigolactone GR24 on Arabidopsis root system was evaluated. 242 ecotypes of Arabidopsis thaliana were analyzed by genome-wide association study (GWAS) under theinfluence of 100 nM of strigolactone. Results of the study are analyzed for a search of candidate genes which participate in strigolactone signaling pathways. From obtained results we have chosen 5 candidate genes (AFB4, F-box, BEN2, F-box associated, MPK) for analysis of their expression levels dependent on strigolactone concentration in Arabidopsis roots and simultaneously auxin efflux (PIN1,2,3,4,7) and influx (AUX1) carriers were analyzed by Real-Time PCR.

Druh

D - Stať ve sborníku

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2014: Proceedings of International Ph.D. Students Conference

ISBN

978-80-7509-174-1

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

447-452

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

19. 11. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000362518300085