Porcine EEF1A1 and EEF1A2 genes: genomic structure, polymorphism, mapping and expression
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906475" target="_blank" >RIV/62156489:43210/15:43906475 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/67985904:_____/15:00446383 RIV/60460709:41210/15:69132
Výsledek na webu
<a href="http://dx.doi.org/10.1007/s11033-015-3866-x" target="_blank" >http://dx.doi.org/10.1007/s11033-015-3866-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11033-015-3866-x" target="_blank" >10.1007/s11033-015-3866-x</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Porcine EEF1A1 and EEF1A2 genes: genomic structure, polymorphism, mapping and expression
Popis výsledku v původním jazyce
Eukaryotic translation elongation factor 1 alpha (EEF1A) plays a key role in protein synthesis. In higher vertebrates EEF1A occurs in two isoforms, EEF1A1 and EEF1A2, encoded by distinct genes. The purpose of this study was to compare the two porcine genes as for the genomic sequence, gene organization and mRNA expression in different tissues, as well as to search for polymorphism and chromosomal assignment. Standard methods of DNA and mRNA analysis were used. We determined the complete genomic sequenceof the porcine EEF1A1 and EEF1A2 genes. The two genes differ in the lengths of transcription units (3102 and 8588 bp, respectively), but have similar genomic organization and their coding sequences are highly similar (78 % identity of coding sequences and 92.4 % identity of amino acid sequences). Several polymorphisms in the two genes were detected. EEF1A1 and EEF1A2 were mapped to SSC1p11.1 and SSC17q23.3, respectively. mRNA of EEF1A1 was expressed in all studied tissues (the highest e
Název v anglickém jazyce
Porcine EEF1A1 and EEF1A2 genes: genomic structure, polymorphism, mapping and expression
Popis výsledku anglicky
Eukaryotic translation elongation factor 1 alpha (EEF1A) plays a key role in protein synthesis. In higher vertebrates EEF1A occurs in two isoforms, EEF1A1 and EEF1A2, encoded by distinct genes. The purpose of this study was to compare the two porcine genes as for the genomic sequence, gene organization and mRNA expression in different tissues, as well as to search for polymorphism and chromosomal assignment. Standard methods of DNA and mRNA analysis were used. We determined the complete genomic sequenceof the porcine EEF1A1 and EEF1A2 genes. The two genes differ in the lengths of transcription units (3102 and 8588 bp, respectively), but have similar genomic organization and their coding sequences are highly similar (78 % identity of coding sequences and 92.4 % identity of amino acid sequences). Several polymorphisms in the two genes were detected. EEF1A1 and EEF1A2 were mapped to SSC1p11.1 and SSC17q23.3, respectively. mRNA of EEF1A1 was expressed in all studied tissues (the highest e
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Molecular Biology Reports
ISSN
0301-4851
e-ISSN
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Svazek periodika
42
Číslo periodika v rámci svazku
8
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
8
Strana od-do
1257-1264
Kód UT WoS článku
000358165300002
EID výsledku v databázi Scopus
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