The effect of metal ions on Staphylococcus aureus revealed by biochemical and mass spectrometric analyses
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43907656" target="_blank" >RIV/62156489:43210/15:43907656 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216305:26620/15:PU113298
Výsledek na webu
<a href="http://www.sciencedirect.com/science/article/pii/S0944501314000895" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0944501314000895</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.micres.2014.08.003" target="_blank" >10.1016/j.micres.2014.08.003</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The effect of metal ions on Staphylococcus aureus revealed by biochemical and mass spectrometric analyses
Popis výsledku v původním jazyce
In this study, we focused on the effect of heavy metal ions in resistant strains of gram-positive bacteria Staphylococcus aureus using biochemical methods and mass spectrometry. Five nitrate solutions of heavy metals (Ag+, Cu2+, Cd2+, Zn2+ and Pb2+) were used to create S. aureus resistant strains. Biochemical changes of resistant strains in comparison with the non-resistant control strain of S. aureus were observed by microbiological (measuring - growth curves and inhibition zones) and spectrophotometric methods (antioxidant activity and alaninaminotransferase, aspartateaminotransferase, alkaline phosphatase, gamma-glutamyltransferase activities). Mass spectrometry was employed for the qualitative analysis of the samples (changes in S. aureus protein composition) and for the identification of the strains database MALDI Biotyper was employed. Alterations, in terms of biochemical properties and protein composition, were observed in resistant strains compared to non-resistant control strain. Our results describe the possible option for the analysis of S. aureus resistant strains and may thus serve as a support for monitoring of changes in genetic information caused by the forming of resistance to heavy metals.
Název v anglickém jazyce
The effect of metal ions on Staphylococcus aureus revealed by biochemical and mass spectrometric analyses
Popis výsledku anglicky
In this study, we focused on the effect of heavy metal ions in resistant strains of gram-positive bacteria Staphylococcus aureus using biochemical methods and mass spectrometry. Five nitrate solutions of heavy metals (Ag+, Cu2+, Cd2+, Zn2+ and Pb2+) were used to create S. aureus resistant strains. Biochemical changes of resistant strains in comparison with the non-resistant control strain of S. aureus were observed by microbiological (measuring - growth curves and inhibition zones) and spectrophotometric methods (antioxidant activity and alaninaminotransferase, aspartateaminotransferase, alkaline phosphatase, gamma-glutamyltransferase activities). Mass spectrometry was employed for the qualitative analysis of the samples (changes in S. aureus protein composition) and for the identification of the strains database MALDI Biotyper was employed. Alterations, in terms of biochemical properties and protein composition, were observed in resistant strains compared to non-resistant control strain. Our results describe the possible option for the analysis of S. aureus resistant strains and may thus serve as a support for monitoring of changes in genetic information caused by the forming of resistance to heavy metals.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CG - Elektrochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/EE2.4.31.0023" target="_blank" >EE2.4.31.0023: Partnerská síť centra excelentního bionanotechnologického výzkumu</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Microbiological Research
ISSN
0944-5013
e-ISSN
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Svazek periodika
170
Číslo periodika v rámci svazku
January
Stát vydavatele periodika
DE - Spolková republika Německo
Počet stran výsledku
10
Strana od-do
147-156
Kód UT WoS článku
000347502500017
EID výsledku v databázi Scopus
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