Effect of mercury on porcine ovarian granulosa cells in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43907987" target="_blank" >RIV/62156489:43210/15:43907987 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41320/15:67386

Výsledek na webu

<a href="http://dx.doi.org/10.1080/10934529.2015.1019805" target="_blank" >http://dx.doi.org/10.1080/10934529.2015.1019805</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/10934529.2015.1019805" target="_blank" >10.1080/10934529.2015.1019805</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of mercury on porcine ovarian granulosa cells in vitro

Popis výsledku v původním jazyce

The objective of this in vitro study was to examine dose-dependent changes in the secretion activity [progesterone (P-4) and insulin-like growth factor-I (IGF-I)] of porcine ovarian granulosa cells after experimental mercury (Hg) administration, including its apoptotic potential so as to ascertain the possible involvement of Hg in steroidogenesis. Ovarian granulosa cells were incubated with mercuric chloride [mercury (II) chloride or HgCl2] at the doses 50-250 mu g mL(-1) for 18h and compared with control group without Hg addition. Release of P-4 and IGF-I by ovarian granulosa cells was assessed by RIA and apoptosis by TUNEL assay. Observations show that P-4 release by granulosa cells was significantly (P < 0.05) inhibited at all the doses, while IGF-Irelease was not affected at any of the doses used, although a decreasing trend in the release of IGF-I was noted in comparison to control. An increasing trend of apoptosis of granulosa cells was noted, the difference being significant (P

Název v anglickém jazyce

Effect of mercury on porcine ovarian granulosa cells in vitro

Popis výsledku anglicky

The objective of this in vitro study was to examine dose-dependent changes in the secretion activity [progesterone (P-4) and insulin-like growth factor-I (IGF-I)] of porcine ovarian granulosa cells after experimental mercury (Hg) administration, including its apoptotic potential so as to ascertain the possible involvement of Hg in steroidogenesis. Ovarian granulosa cells were incubated with mercuric chloride [mercury (II) chloride or HgCl2] at the doses 50-250 mu g mL(-1) for 18h and compared with control group without Hg addition. Release of P-4 and IGF-I by ovarian granulosa cells was assessed by RIA and apoptosis by TUNEL assay. Observations show that P-4 release by granulosa cells was significantly (P < 0.05) inhibited at all the doses, while IGF-Irelease was not affected at any of the doses used, although a decreasing trend in the release of IGF-I was noted in comparison to control. An increasing trend of apoptosis of granulosa cells was noted, the difference being significant (P

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Environmental Science and Health. Part A-Toxic Hazardous Substances &amp; Environmental Engineering

ISSN

1093-4529

e-ISSN

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Svazek periodika

50

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

839-845

Kód UT WoS článku

000355407700008

EID výsledku v databázi Scopus

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