Characterisation of Magnetic Particles - DNA Interaction for Imobilisation of Viruses and Their Subsequent Identification by Zeta Potential and Particle Size Analyses

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43908591" target="_blank" >RIV/62156489:43210/15:43908591 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/15:PU126353

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Characterisation of Magnetic Particles - DNA Interaction for Imobilisation of Viruses and Their Subsequent Identification by Zeta Potential and Particle Size Analyses

Popis výsledku v původním jazyce

Magnetic nanoparticles offer several advantages for isolation of biological materials. Since they are broadly used in diagnostic immunoassays and in various reactions involving enzymes, proteins, and DNA, this study considers the interaction of four surface modified magnetic microparticles with selected DNA fragments. The magnetic microparticle precursors are prepared by reduction of from iron (III) nitrate and sodium borohydride in ammonia solution. Different surface modifications, named as MAN 37, MAN127, MAN 158 and MAN 164 were obtained by targeted chemical treatments. The zeta potential and particle size measurements were performed in a zetasizer MALVERN instrument. All considered magnetic microparticles are characterized by positive zeta-potentials, while the DNA fragments in solution show an approx. zeta-potential of -0.22 mV. All the particles were polycharged. MAN 37 and MAN 127 reveal the highest initial zeta potentials. Upon exposure to DNA, MAN 37 and MAN 127 particles low

Název v anglickém jazyce

Characterisation of Magnetic Particles - DNA Interaction for Imobilisation of Viruses and Their Subsequent Identification by Zeta Potential and Particle Size Analyses

Popis výsledku anglicky

Magnetic nanoparticles offer several advantages for isolation of biological materials. Since they are broadly used in diagnostic immunoassays and in various reactions involving enzymes, proteins, and DNA, this study considers the interaction of four surface modified magnetic microparticles with selected DNA fragments. The magnetic microparticle precursors are prepared by reduction of from iron (III) nitrate and sodium borohydride in ammonia solution. Different surface modifications, named as MAN 37, MAN127, MAN 158 and MAN 164 were obtained by targeted chemical treatments. The zeta potential and particle size measurements were performed in a zetasizer MALVERN instrument. All considered magnetic microparticles are characterized by positive zeta-potentials, while the DNA fragments in solution show an approx. zeta-potential of -0.22 mV. All the particles were polycharged. MAN 37 and MAN 127 reveal the highest initial zeta potentials. Upon exposure to DNA, MAN 37 and MAN 127 particles low

Druh

D - Stať ve sborníku

CEP obor

CC - Organická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NANOCON 2015: Conference Proceedings

ISBN

978-80-87294-63-5

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

505-510

Název nakladatele

Tanger Ltd.

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

14. 10. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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