Detection of DNA fragments from wheat in blood of animals
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43909622" target="_blank" >RIV/62156489:43210/16:43909622 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1007/s00003-016-1035-3" target="_blank" >http://dx.doi.org/10.1007/s00003-016-1035-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00003-016-1035-3" target="_blank" >10.1007/s00003-016-1035-3</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Detection of DNA fragments from wheat in blood of animals
Popis výsledku v původním jazyce
Delivery of foreign DNA is still a very controversial issue, especially in relation to genetically modified organisms. It is not necessary to use genetically modified feed to verify the possible transfer of DNA from food into the body. In this study, we evaluated the possible transfer of DNA from wheat forage (of single component diet) into the blood of three different organisms. DNA isolation kits were used to isolate total DNA from blood and feed. Blood was taken for analysis from rats (after 23 days feeding wheat), broilers (15 days), and carp (34 days). Housekeeping genes were used to verify blood samples (rats: glyceraldehyde-3-phosphate dehydrogenase/GAPDH/, broilers: growth hormone gene/GCH/and carp: small ribosomal subunit/40S/). We used GAPDH and actin to determine whether DNA from wheat was transferred into animal blood. No purple and common wheat DNA fragments were detected in the blood of any animal tested, suggesting no transfer of the tested wheat DNA into the body. Various contents of anthocyanins in the feeding variants had no effect on the transfer of wheat DNA into the blood of animals. Our results correspond to some studies that have not proved transfer DNA from genetically modified feed into the target organism.
Název v anglickém jazyce
Detection of DNA fragments from wheat in blood of animals
Popis výsledku anglicky
Delivery of foreign DNA is still a very controversial issue, especially in relation to genetically modified organisms. It is not necessary to use genetically modified feed to verify the possible transfer of DNA from food into the body. In this study, we evaluated the possible transfer of DNA from wheat forage (of single component diet) into the blood of three different organisms. DNA isolation kits were used to isolate total DNA from blood and feed. Blood was taken for analysis from rats (after 23 days feeding wheat), broilers (15 days), and carp (34 days). Housekeeping genes were used to verify blood samples (rats: glyceraldehyde-3-phosphate dehydrogenase/GAPDH/, broilers: growth hormone gene/GCH/and carp: small ribosomal subunit/40S/). We used GAPDH and actin to determine whether DNA from wheat was transferred into animal blood. No purple and common wheat DNA fragments were detected in the blood of any animal tested, suggesting no transfer of the tested wheat DNA into the body. Various contents of anthocyanins in the feeding variants had no effect on the transfer of wheat DNA into the blood of animals. Our results correspond to some studies that have not proved transfer DNA from genetically modified feed into the target organism.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
—
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal für Verbraucherschutz und Lebensmittelsicherheit
ISSN
1661-5751
e-ISSN
—
Svazek periodika
11
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
6
Strana od-do
259-264
Kód UT WoS článku
000382704600006
EID výsledku v databázi Scopus
2-s2.0-84976439951