Generation of Arabidopsis Lines with Altered Cytokinin Level Expressing GFP-Fused Cytoskeletal Proteins

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43910205" target="_blank" >RIV/62156489:43210/16:43910205 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2016/mnet_2016_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2016/mnet_2016_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Generation of Arabidopsis Lines with Altered Cytokinin Level Expressing GFP-Fused Cytoskeletal Proteins

Popis výsledku v původním jazyce

Cell division, expansion and differentiation require a sophisticated spatial arrangement of the cytoskeleton. The ever increasing progress in fluorescent microscope techniques has allowed to visualize rearrangements and dynamics of actin and microtubule arrays in various cell types and tissues in plants. Processes controlling proper cytoskeleton organisation and its response to various stimuli, mediated by actin and microtubule binding proteins as well as plant hormones, are being an area of active investigation. As the current research of cytoskeleton regulation focused mainly on hormone auxin, the involvement of cytokinin is still unclear. In this study, we describe generation of transgenic lines of Arabidopsis thaliana comprising inducible system for manipulation of endogenous cytokinin level and simultaneously GFP-labelled actin or microtubule proteins. These lines serve as a tool for the study of cytokinin mode of action in the course of cell/organ development. Based on our results, it is obvious that cytokinins play a considerable role in modulation of plant cytoskeleton. However, further research is necessary to elucidate precise cytokinin signalling pathways and cross-talk with other hormones, which participate in such complex processes of cell morphology.

Název v anglickém jazyce

Generation of Arabidopsis Lines with Altered Cytokinin Level Expressing GFP-Fused Cytoskeletal Proteins

Popis výsledku anglicky

Cell division, expansion and differentiation require a sophisticated spatial arrangement of the cytoskeleton. The ever increasing progress in fluorescent microscope techniques has allowed to visualize rearrangements and dynamics of actin and microtubule arrays in various cell types and tissues in plants. Processes controlling proper cytoskeleton organisation and its response to various stimuli, mediated by actin and microtubule binding proteins as well as plant hormones, are being an area of active investigation. As the current research of cytoskeleton regulation focused mainly on hormone auxin, the involvement of cytokinin is still unclear. In this study, we describe generation of transgenic lines of Arabidopsis thaliana comprising inducible system for manipulation of endogenous cytokinin level and simultaneously GFP-labelled actin or microtubule proteins. These lines serve as a tool for the study of cytokinin mode of action in the course of cell/organ development. Based on our results, it is obvious that cytokinins play a considerable role in modulation of plant cytoskeleton. However, further research is necessary to elucidate precise cytokinin signalling pathways and cross-talk with other hormones, which participate in such complex processes of cell morphology.

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2016: Proceedings of International PhD Students Conference

ISBN

978-80-7509-443-8

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

757-762

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

9. 11. 2016

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000392968500135