Determination of S-Adenosylmethionine and S-Adenosylhomocysteine in Two Prostatic Adenocarcinoma Cell Lines

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43910232" target="_blank" >RIV/62156489:43210/16:43910232 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/16:PU122568

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2016/mnet_2016_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2016/mnet_2016_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of S-Adenosylmethionine and S-Adenosylhomocysteine in Two Prostatic Adenocarcinoma Cell Lines

Popis výsledku v původním jazyce

Studies of processes leading to cancer tumour progression are one of the major foci of current cancer research. Therefore, it is important to shade the light on the metabolic pathways involved in cancerogenesis. In the present study we have chosen as a model two prostatic adenocarcinoma cell lines (PC-3 and LNCaP) for the comparison of the metabolites involved in tumour growth. Applying the HPLC-MS method, we determined and compared the concentration levels of S-(5'-adenosyl)-L-homocysteine (SAH) and S-(5'-adenosyl)-L-methionine (SAM), that are involved in the tumour growth processes. SAM/SAH ratio, a methylation index describing the aggressiveness of tumour cells, was determined here. The methylation index was 0.4 and 1.2 for PC-3 and LNCaP cells, respectively, pointing out that the PC-3 cells are more aggressive than LNCaP cells. This finding is of great importance for our following studies.

Název v anglickém jazyce

Determination of S-Adenosylmethionine and S-Adenosylhomocysteine in Two Prostatic Adenocarcinoma Cell Lines

Popis výsledku anglicky

Studies of processes leading to cancer tumour progression are one of the major foci of current cancer research. Therefore, it is important to shade the light on the metabolic pathways involved in cancerogenesis. In the present study we have chosen as a model two prostatic adenocarcinoma cell lines (PC-3 and LNCaP) for the comparison of the metabolites involved in tumour growth. Applying the HPLC-MS method, we determined and compared the concentration levels of S-(5'-adenosyl)-L-homocysteine (SAH) and S-(5'-adenosyl)-L-methionine (SAM), that are involved in the tumour growth processes. SAM/SAH ratio, a methylation index describing the aggressiveness of tumour cells, was determined here. The methylation index was 0.4 and 1.2 for PC-3 and LNCaP cells, respectively, pointing out that the PC-3 cells are more aggressive than LNCaP cells. This finding is of great importance for our following studies.

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA16-18917S" target="_blank" >GA16-18917S: Studium metabolismu sarkosinu a jeho participace na vývoji nádorů prostaty</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2016: Proceedings of International PhD Students Conference

ISBN

978-80-7509-443-8

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

972-977

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

9. 11. 2016

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000392968500174