Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43911543" target="_blank" >RIV/62156489:43210/17:43911543 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/17:PU123082

Výsledek na webu

<a href="https://doi.org/10.2147/IJN.S121840" target="_blank" >https://doi.org/10.2147/IJN.S121840</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2147/IJN.S121840" target="_blank" >10.2147/IJN.S121840</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA

Popis výsledku v původním jazyce

CdTe/ZnSe core/shell quantum dot (QD), one of the strongest and most highly luminescent nanoparticles, was directly synthesized in an aqueous medium to study its individual interactions with important nucleobases (adenine, guanine, cytosine, and thymine) in detail. The results obtained from the optical analyses indicated that the interactions of the QDs with different nucleobases were different, which reflected in different fluorescent emission maxima and intensities. The difference in the interaction was found due to the different chemical behavior and different sizes of the formed nanoconjugates. An electrochemical study also confirmed that the purines and pyrimidines show different interactions with the core/shell QDs. Based on these phenomena, a novel QD-based method is developed to detect the presence of the DNA, damage to DNA, and mutation. The QDs were successfully applied very easily to detect any change in the sequence (mutation) of DNA. The QDs also showed their ability to detect DNAs directly from the extracts of human cancer (PC3) and normal (PNT1A) cells (detection limit of 500 pM of DNA), which indicates the possibilities to use this easy assay technique to confirm the presence of living organisms in extreme environments.

Název v anglickém jazyce

Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA

Popis výsledku anglicky

CdTe/ZnSe core/shell quantum dot (QD), one of the strongest and most highly luminescent nanoparticles, was directly synthesized in an aqueous medium to study its individual interactions with important nucleobases (adenine, guanine, cytosine, and thymine) in detail. The results obtained from the optical analyses indicated that the interactions of the QDs with different nucleobases were different, which reflected in different fluorescent emission maxima and intensities. The difference in the interaction was found due to the different chemical behavior and different sizes of the formed nanoconjugates. An electrochemical study also confirmed that the purines and pyrimidines show different interactions with the core/shell QDs. Based on these phenomena, a novel QD-based method is developed to detect the presence of the DNA, damage to DNA, and mutation. The QDs were successfully applied very easily to detect any change in the sequence (mutation) of DNA. The QDs also showed their ability to detect DNAs directly from the extracts of human cancer (PC3) and normal (PNT1A) cells (detection limit of 500 pM of DNA), which indicates the possibilities to use this easy assay technique to confirm the presence of living organisms in extreme environments.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Nanomedicine

ISSN

1176-9114

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

14 February

Stát vydavatele periodika

NZ - Nový Zéland

Počet stran výsledku

15

Strana od-do

1277-1291

Kód UT WoS článku

000394047900003

EID výsledku v databázi Scopus

2-s2.0-85013187207