Long-term impact of cadmium shows little damage in Scenedesmus acutiformis cultures

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43911845" target="_blank" >RIV/62156489:43210/17:43911845 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/17:00097503 RIV/62157124:16370/17:43875675

Výsledek na webu

<a href="https://doi.org/10.1016/j.algal.2017.04.029" target="_blank" >https://doi.org/10.1016/j.algal.2017.04.029</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.algal.2017.04.029" target="_blank" >10.1016/j.algal.2017.04.029</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Long-term impact of cadmium shows little damage in Scenedesmus acutiformis cultures

Popis výsledku v původním jazyce

Long-term (30 days) responses of green alga Scenedesmus acutiformis to cadmium (Cd) excess (1 or 10 mu M) were studied under in vitro heterotrophic conditions (Milieu Bristol medium containing glucose and casein). No extensive changes of morphology were observed but Cd slightly depleted chlorophyll autofluorescence and some mineral content (K, Ca, and Mg). However, Cd (mainly at 10 mu M) stimulated increase in biomass and accumulation of ascorbic acid, non-protein thiols, and phenols. Signs of oxidative stress (elevation of ROS, depletion of nitric oxide and viability, changes in the activities of SOD, CAT and GR) were also observed. Total Cd reached only 17.3 and 45.2 mu g/g DW at 1 or 10 mu M Cd but bioaccumulation factor (BAF) was comparable with other species and cultivation/exposure conditions (BAF similar to 40 at 10 mu M Cd) indicating that rather inorganic compounds than glucose or casein affect Cd accumulation. Short-term (24 h) exposure of algae to identical Cd doses in HEPES buffer revealed higher absolute Cd content in comparison with 30 days of cultivation (8-and 17fold) and comparison with previous studies indicates that S. acutiformis is a better cadmium accumulator. The extensive use of fluorescence microscopy for oxidative stress detection in algae is also carefully discussed.

Název v anglickém jazyce

Long-term impact of cadmium shows little damage in Scenedesmus acutiformis cultures

Popis výsledku anglicky

Long-term (30 days) responses of green alga Scenedesmus acutiformis to cadmium (Cd) excess (1 or 10 mu M) were studied under in vitro heterotrophic conditions (Milieu Bristol medium containing glucose and casein). No extensive changes of morphology were observed but Cd slightly depleted chlorophyll autofluorescence and some mineral content (K, Ca, and Mg). However, Cd (mainly at 10 mu M) stimulated increase in biomass and accumulation of ascorbic acid, non-protein thiols, and phenols. Signs of oxidative stress (elevation of ROS, depletion of nitric oxide and viability, changes in the activities of SOD, CAT and GR) were also observed. Total Cd reached only 17.3 and 45.2 mu g/g DW at 1 or 10 mu M Cd but bioaccumulation factor (BAF) was comparable with other species and cultivation/exposure conditions (BAF similar to 40 at 10 mu M Cd) indicating that rather inorganic compounds than glucose or casein affect Cd accumulation. Short-term (24 h) exposure of algae to identical Cd doses in HEPES buffer revealed higher absolute Cd content in comparison with 30 days of cultivation (8-and 17fold) and comparison with previous studies indicates that S. acutiformis is a better cadmium accumulator. The extensive use of fluorescence microscopy for oxidative stress detection in algae is also carefully discussed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Algal Research-Biology, Biomass and Biofuels

ISSN

2211-9264

e-ISSN

—

Svazek periodika

25

Číslo periodika v rámci svazku

July

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

184-190

Kód UT WoS článku

000406305900022

EID výsledku v databázi Scopus

2-s2.0-85019648612