DNA interaction with platinum-based cytostatics revealed by DNA sequencing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912469" target="_blank" >RIV/62156489:43210/17:43912469 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/17:00100210 RIV/00216208:11130/17:10369484 RIV/00216208:11310/17:10369484 RIV/00216305:26620/17:PU125955 RIV/00064203:_____/17:10369484

Výsledek na webu

<a href="https://doi.org/10.1016/j.ab.2017.09.018" target="_blank" >https://doi.org/10.1016/j.ab.2017.09.018</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ab.2017.09.018" target="_blank" >10.1016/j.ab.2017.09.018</a>

Jazyk výsledku

angličtina

Název v původním jazyce

DNA interaction with platinum-based cytostatics revealed by DNA sequencing

Popis výsledku v původním jazyce

The main mechanism of action of platinum-based cytostatic drugs - cisplatin, oxaliplatin and carboplatin - is the formation of DNA cross-links, which restricts the transcription due to the disability of DNA to enter the active site of the polymerase. The polymerase chain reaction (PCR) was employed as a simplified model of the amplification process in the cell nucleus. PCR with fluorescently labelled dideoxynucleotides commonly employed for DNA sequencing was used to monitor the effect of platinum-based cytostatics on DNA in terms of decrease in labeling efficiency dependent on a presence of the DNA-drug cross-link. It was found that significantly different amounts of the drugs - cisplatin (0.21 μg/mL), oxaliplatin (5.23 μg/mL), and carboplatin (71.11 μg/mL) - were required to cause the same quenching effect (50%) on the fluorescent labelling of 50 μg/mL of DNA. Moreover, it was found that even though the amounts of the drugs was applied to the reaction mixture differing by several orders of magnitude, the amount of incorporated platinum, quantified by inductively coupled plasma mass spectrometry, was in all cases at the level of tenths of μg per 5 μg of DNA.

Název v anglickém jazyce

DNA interaction with platinum-based cytostatics revealed by DNA sequencing

Popis výsledku anglicky

The main mechanism of action of platinum-based cytostatic drugs - cisplatin, oxaliplatin and carboplatin - is the formation of DNA cross-links, which restricts the transcription due to the disability of DNA to enter the active site of the polymerase. The polymerase chain reaction (PCR) was employed as a simplified model of the amplification process in the cell nucleus. PCR with fluorescently labelled dideoxynucleotides commonly employed for DNA sequencing was used to monitor the effect of platinum-based cytostatics on DNA in terms of decrease in labeling efficiency dependent on a presence of the DNA-drug cross-link. It was found that significantly different amounts of the drugs - cisplatin (0.21 μg/mL), oxaliplatin (5.23 μg/mL), and carboplatin (71.11 μg/mL) - were required to cause the same quenching effect (50%) on the fluorescent labelling of 50 μg/mL of DNA. Moreover, it was found that even though the amounts of the drugs was applied to the reaction mixture differing by several orders of magnitude, the amount of incorporated platinum, quantified by inductively coupled plasma mass spectrometry, was in all cases at the level of tenths of μg per 5 μg of DNA.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Biochemistry

ISSN

0003-2697

e-ISSN

—

Svazek periodika

539

Číslo periodika v rámci svazku

15 December

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

22-28

Kód UT WoS článku

000417117000004

EID výsledku v databázi Scopus

2-s2.0-85030719997