The effect of coffee supplementation on glutathione and total thiols levels

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912621" target="_blank" >RIV/62156489:43210/17:43912621 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/17:PU128347

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effect of coffee supplementation on glutathione and total thiols levels

Popis výsledku v původním jazyce

Antioxidants are very important substances that counteract the formation of free radicals. They are divided into exogenous, such as vitamin C which the body receives with food, and endogenous. One of the most important endogenous antioxidants is glutathione (γ-glutamyl-L-cysteinyl-glycine), which plays an important role in cellular defense against oxidative damage. Free glutathione is presented within organisms in both reduced (GSH) and oxidized forms (GSSG). Oxidative stress leads to a decrease in GSH level and therefore a GSH/GSSG ratio that can be used as an indicator of oxidative stress and an indicator of various diseases. The aim of presented study was to develop a sampling method for capillary blood testing, where we have found that this amount (15 µl) is sufficient for our testing. Another aim was to supplement group of volunteers with coffee and to determine GSH levels and levels of total thiols after 0, 48 h and 96 h of supplementation in capillary blood. HPLC with electrochemical detection was used for GSH determination and Ellman&apos;s method for determination of total thiols. We could see the GSH slight increase as well as the levels of total thiols.

Název v anglickém jazyce

The effect of coffee supplementation on glutathione and total thiols levels

Popis výsledku anglicky

Antioxidants are very important substances that counteract the formation of free radicals. They are divided into exogenous, such as vitamin C which the body receives with food, and endogenous. One of the most important endogenous antioxidants is glutathione (γ-glutamyl-L-cysteinyl-glycine), which plays an important role in cellular defense against oxidative damage. Free glutathione is presented within organisms in both reduced (GSH) and oxidized forms (GSSG). Oxidative stress leads to a decrease in GSH level and therefore a GSH/GSSG ratio that can be used as an indicator of oxidative stress and an indicator of various diseases. The aim of presented study was to develop a sampling method for capillary blood testing, where we have found that this amount (15 µl) is sufficient for our testing. Another aim was to supplement group of volunteers with coffee and to determine GSH levels and levels of total thiols after 0, 48 h and 96 h of supplementation in capillary blood. HPLC with electrochemical detection was used for GSH determination and Ellman&apos;s method for determination of total thiols. We could see the GSH slight increase as well as the levels of total thiols.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2017: Proceedings of International PhD Students Conference

ISBN

978-80-7509-529-9

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

6

Strana od-do

843-848

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

8. 11. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000440194500151