Kinetic analysis of human metallothionein and CdTe quantum dot complexes using fluorescence and voltammetry techniques

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912864" target="_blank" >RIV/62156489:43210/17:43912864 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/17:PU124985

Výsledek na webu

<a href="https://doi.org/10.1016/j.colsurfb.2017.09.033" target="_blank" >https://doi.org/10.1016/j.colsurfb.2017.09.033</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.colsurfb.2017.09.033" target="_blank" >10.1016/j.colsurfb.2017.09.033</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Kinetic analysis of human metallothionein and CdTe quantum dot complexes using fluorescence and voltammetry techniques

Popis výsledku v původním jazyce

Thanks to quantum dots&apos; (QDs) properties, they can be used as selective and sensitive biomarkers in molecular imaging. In a previous paper, we confirmed the possibility of interaction between mercaptosuccinic acid-capped cadmium telluride QDs (MSA-CdTe) and human metallothionein (MT). The aim of this study was to expand on our previous research with an evaluation of the stability of the formed complexes between human MT and four CdTe compounds of the following sizes: 3.4 nm (blue QDs), 3.8 nm (green QDs), 4.5 nm (yellow QDs), and 5.2 nm (red QDs). Complexes were evaluated over time using fluorescence intensity and differential pulse voltammetry. Differences between the voltammograms obtained for standard solutions and for CdTe + MT show that complexes were formed. An increase in fluorescence intensity was observed for blue (Delta%approximate to 40 for t = 1 -&gt; 120 min) and red (Delta%approximate to 30 for t = 1 -&gt; 120 min) CdTe-MT complexes than CdTe alone, whereas green and yellow CdTe-MT complexes had a lower fluorescence intensity than CdTe alone. A stronger time dependence of the mercaptosuccinic acid (MSA) peak height on the timeline and differences in the MSA peak shape (in CdTe, and CdTe + MT complexes) were also observed by voltammetry. Authors noticed a decrease in the Cat2 signal of the red and green CdTe + MT complexes at the time of conjugation. Our results reveal that the size of QDs has an impact on the interaction between CdTe and human MT, as well as on the stability of complexes formed during these interactions. The bioconjugates&apos; stability was also found to depend on the time of interaction.

Název v anglickém jazyce

Kinetic analysis of human metallothionein and CdTe quantum dot complexes using fluorescence and voltammetry techniques

Popis výsledku anglicky

Thanks to quantum dots&apos; (QDs) properties, they can be used as selective and sensitive biomarkers in molecular imaging. In a previous paper, we confirmed the possibility of interaction between mercaptosuccinic acid-capped cadmium telluride QDs (MSA-CdTe) and human metallothionein (MT). The aim of this study was to expand on our previous research with an evaluation of the stability of the formed complexes between human MT and four CdTe compounds of the following sizes: 3.4 nm (blue QDs), 3.8 nm (green QDs), 4.5 nm (yellow QDs), and 5.2 nm (red QDs). Complexes were evaluated over time using fluorescence intensity and differential pulse voltammetry. Differences between the voltammograms obtained for standard solutions and for CdTe + MT show that complexes were formed. An increase in fluorescence intensity was observed for blue (Delta%approximate to 40 for t = 1 -&gt; 120 min) and red (Delta%approximate to 30 for t = 1 -&gt; 120 min) CdTe-MT complexes than CdTe alone, whereas green and yellow CdTe-MT complexes had a lower fluorescence intensity than CdTe alone. A stronger time dependence of the mercaptosuccinic acid (MSA) peak height on the timeline and differences in the MSA peak shape (in CdTe, and CdTe + MT complexes) were also observed by voltammetry. Authors noticed a decrease in the Cat2 signal of the red and green CdTe + MT complexes at the time of conjugation. Our results reveal that the size of QDs has an impact on the interaction between CdTe and human MT, as well as on the stability of complexes formed during these interactions. The bioconjugates&apos; stability was also found to depend on the time of interaction.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Colloids and Surfaces B. Biointerfaces

ISSN

0927-7765

e-ISSN

—

Svazek periodika

160

Číslo periodika v rámci svazku

1 December

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

381-389

Kód UT WoS článku

000418978500046

EID výsledku v databázi Scopus

2-s2.0-85030109152