Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43913349" target="_blank" >RIV/62156489:43210/18:43913349 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/18:00102718 RIV/00216305:26620/18:PU127621

Výsledek na webu

<a href="https://doi.org/10.1016/j.snb.2017.11.074" target="_blank" >https://doi.org/10.1016/j.snb.2017.11.074</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2017.11.074" target="_blank" >10.1016/j.snb.2017.11.074</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection

Popis výsledku v původním jazyce

Human papillomavirus (HPV) and human immunodeficiency virus (HIV) are closely related infections; both diseases are mutually reinforcing due to the co-infection effects, which increase their clinical and epidemiological implications, raising urgent public health concerns. In the present study, a nano-system was developed for simultaneous detection of HPV and HIV targeting E6-HPV16 and Gag-HIV genes respectively. The target sequences from the samples of the patients suffering from head and neck cancer were isolated by magnetic glass particles (MGPs) and subsequently were hybridized with a mixture of the complementary probes attached to cadmium telluride quantum dots (CdTe QDs). Finally, the specific and successful binding was determined by an easy fluorescence analyses and was corroborated by PCR and electrochemistry. Red and green CdTe QDs were used for the dual-color detection of HPV and HIV respectively. Our nano-system can be applied as a proficient tool for the identification of HPV, HIV or both diseases simultaneously, getting a successful and high sensitivity system of detection.

Název v anglickém jazyce

Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection

Popis výsledku anglicky

Human papillomavirus (HPV) and human immunodeficiency virus (HIV) are closely related infections; both diseases are mutually reinforcing due to the co-infection effects, which increase their clinical and epidemiological implications, raising urgent public health concerns. In the present study, a nano-system was developed for simultaneous detection of HPV and HIV targeting E6-HPV16 and Gag-HIV genes respectively. The target sequences from the samples of the patients suffering from head and neck cancer were isolated by magnetic glass particles (MGPs) and subsequently were hybridized with a mixture of the complementary probes attached to cadmium telluride quantum dots (CdTe QDs). Finally, the specific and successful binding was determined by an easy fluorescence analyses and was corroborated by PCR and electrochemistry. Red and green CdTe QDs were used for the dual-color detection of HPV and HIV respectively. Our nano-system can be applied as a proficient tool for the identification of HPV, HIV or both diseases simultaneously, getting a successful and high sensitivity system of detection.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LO1401" target="_blank" >LO1401: Interdisciplinární výzkum bezdrátových technologií</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Sensors and Actuators B. Chemical

ISSN

0925-4005

e-ISSN

—

Svazek periodika

258

Číslo periodika v rámci svazku

1 April

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

295-303

Kód UT WoS článku

000424868700033

EID výsledku v databázi Scopus

2-s2.0-85034805670