Oxidative stress response of rainbow trout (Oncorhynchus mykiss) to multiple stressors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43913864" target="_blank" >RIV/62156489:43210/18:43913864 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60162694:G44__/18:43889526 RIV/62157124:16270/18:43877107

Výsledek na webu

<a href="https://doi.org/10.2754/avb201887010055" target="_blank" >https://doi.org/10.2754/avb201887010055</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb201887010055" target="_blank" >10.2754/avb201887010055</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Oxidative stress response of rainbow trout (Oncorhynchus mykiss) to multiple stressors

Popis výsledku v původním jazyce

Fish can be exposed to multiple stressors at the same time, including natural toxins, environmental pollutants or feed contamination. In this study, we report on the effects of controlled exposure of rainbow trout (Oncorhynchus mykiss) to multiple stressors. In doing so, we test the hypothesis that cyanobacterial biomass and arsenic in feed combine to enhance negative effects on fish, including modulation of antioxidant response. The trout (mean weight 288 +/- 59 g) were divided into six experimental groups of 25 fish each (C - control group; B - 3% lyophilised toxic cyanobacterial biomass; As5 - arsenic at 5 mg.kg(-1); As50 - arsenic at 50 mg.kg(-1); and two combinations of cyanobacterial biomass and arsenic (B + As5 and B + As50). All indicators of oxidative stress were determined using standard methods, showing significant changes. Overall antioxidant capacity (ferric reducing antioxidant power) decreased significantly in the liver, gills and heart after 10-days, while lipid peroxidation (thiobarbituric acid reactive substances) increased in the liver of groups exposed to cyanobacterial biomass, and in the heart of groups exposed to increased concentrations of arsenic and those under combined exposure. Levels of glutathione reductase showed a significant reduction following exposure, especially in the gills and liver. Our results indicate that the antioxidant system plays an important role in the protective response of tissues to stressors, and especially arsenic.

Název v anglickém jazyce

Oxidative stress response of rainbow trout (Oncorhynchus mykiss) to multiple stressors

Popis výsledku anglicky

Fish can be exposed to multiple stressors at the same time, including natural toxins, environmental pollutants or feed contamination. In this study, we report on the effects of controlled exposure of rainbow trout (Oncorhynchus mykiss) to multiple stressors. In doing so, we test the hypothesis that cyanobacterial biomass and arsenic in feed combine to enhance negative effects on fish, including modulation of antioxidant response. The trout (mean weight 288 +/- 59 g) were divided into six experimental groups of 25 fish each (C - control group; B - 3% lyophilised toxic cyanobacterial biomass; As5 - arsenic at 5 mg.kg(-1); As50 - arsenic at 50 mg.kg(-1); and two combinations of cyanobacterial biomass and arsenic (B + As5 and B + As50). All indicators of oxidative stress were determined using standard methods, showing significant changes. Overall antioxidant capacity (ferric reducing antioxidant power) decreased significantly in the liver, gills and heart after 10-days, while lipid peroxidation (thiobarbituric acid reactive substances) increased in the liver of groups exposed to cyanobacterial biomass, and in the heart of groups exposed to increased concentrations of arsenic and those under combined exposure. Levels of glutathione reductase showed a significant reduction following exposure, especially in the gills and liver. Our results indicate that the antioxidant system plays an important role in the protective response of tissues to stressors, and especially arsenic.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/QJ1510077" target="_blank" >QJ1510077: Zvýšení a zefektivnění produkce lososovitých ryb v ČR s využitím jejich genetické identifikace.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Veterinaria Brno

ISSN

0001-7213

e-ISSN

—

Svazek periodika

87

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

55-64

Kód UT WoS článku

000431956300007

EID výsledku v databázi Scopus

2-s2.0-85045403676