Effect of prostate cancer cell line supernatant on functional polarization in macrophages

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43913911" target="_blank" >RIV/62156489:43210/18:43913911 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/18:00104058

Výsledek na webu

<a href="https://doi.org/10.4149/BLL_2018_095" target="_blank" >https://doi.org/10.4149/BLL_2018_095</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4149/BLL_2018_095" target="_blank" >10.4149/BLL_2018_095</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of prostate cancer cell line supernatant on functional polarization in macrophages

Popis výsledku v původním jazyce

OBJECTIVES: We aimed on effect of supernatant derived from prostate cancer cell line PC-3 on M1/M2 functional polarization in macrophages. BACKGROUND: Cytokines play an important role in carcinogenesis. Most of them are produced by macrophages. Macrophages are divided into groups M1 or M2. Classical phenotype macrophages M1 support pro-inflammatory effects and produce pro-infl ammatory cytokines, such as interleukin 6 (IL-6), interleukin 12 (IL-12), tumor necrosis factor α (TNF-α). Macrophages exhibiting a phenotype M2 secrete anti-infl ammatory cytokines, e. g. interleukin 10 (IL-10), transforming growth factor β (TGF-β). METHODS: Peripheral blood monocytes were cultivated for 7 days and during this time went through a differentiation into macrophages. Macrophages were stimulated for 24 hours by lipopolysaccharide (LPS) as a positive control and cultivated with supernatant for another 24 hours. RESULTS: Macrophages cultivated without LPS and without supernatant were used as negative control. Relative expression of IL-6, IL-10, IL-12 and TNF-α was measured by Quantitative real-time PCR. Expression of pro-infl ammatory cytokines was lower in macrophages with supernatant compared to positive control. CONCLUSION: Expression of pro-infl ammatory cytokines was lower in macrophages with supernatant (MΦ+sup) compared to positive control (MΦ+LPS). Effect of the supernatant on expression of IL-6, IL-10, IL-12 and TNF-α was not confi rmed (Tab. 1, Fig. 5, Ref. 15).

Název v anglickém jazyce

Effect of prostate cancer cell line supernatant on functional polarization in macrophages

Popis výsledku anglicky

OBJECTIVES: We aimed on effect of supernatant derived from prostate cancer cell line PC-3 on M1/M2 functional polarization in macrophages. BACKGROUND: Cytokines play an important role in carcinogenesis. Most of them are produced by macrophages. Macrophages are divided into groups M1 or M2. Classical phenotype macrophages M1 support pro-inflammatory effects and produce pro-infl ammatory cytokines, such as interleukin 6 (IL-6), interleukin 12 (IL-12), tumor necrosis factor α (TNF-α). Macrophages exhibiting a phenotype M2 secrete anti-infl ammatory cytokines, e. g. interleukin 10 (IL-10), transforming growth factor β (TGF-β). METHODS: Peripheral blood monocytes were cultivated for 7 days and during this time went through a differentiation into macrophages. Macrophages were stimulated for 24 hours by lipopolysaccharide (LPS) as a positive control and cultivated with supernatant for another 24 hours. RESULTS: Macrophages cultivated without LPS and without supernatant were used as negative control. Relative expression of IL-6, IL-10, IL-12 and TNF-α was measured by Quantitative real-time PCR. Expression of pro-infl ammatory cytokines was lower in macrophages with supernatant compared to positive control. CONCLUSION: Expression of pro-infl ammatory cytokines was lower in macrophages with supernatant (MΦ+sup) compared to positive control (MΦ+LPS). Effect of the supernatant on expression of IL-6, IL-10, IL-12 and TNF-α was not confi rmed (Tab. 1, Fig. 5, Ref. 15).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30204 - Oncology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Bratislavské lekárske listy (Bratislava Medical Journal)

ISSN

0006-9248

e-ISSN

—

Svazek periodika

119

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

6

Strana od-do

516-521

Kód UT WoS článku

000444369100011

EID výsledku v databázi Scopus

2-s2.0-85052808089