Isolation and detail characterization of aba1 T-DNA insertion mutant line of Arabidopsis thaliana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43914674" target="_blank" >RIV/62156489:43210/18:43914674 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isolation and detail characterization of aba1 T-DNA insertion mutant line of Arabidopsis thaliana

Popis výsledku v původním jazyce

Abscisic acid is a plant hormone that affects many biological processes during plant lifespan including stomatal movement, seed and bud dormancy and organ size. Nevertheless, the role of abscisic acid in photomorphogenesis remains rudimentary. To study the role of abscisic acid in different light conditions, the characterization of homozygous mutant lines in biosynthetic gene encoding zeaxanthin epoxidase (ABA1) is essential for genetic studies. Here, we present isolation and characterization of one of the T-DNA insertion mutant lines of Arabidopsis thaliana ecotype Colombia-0, where the insertion is located in 5&apos; UTR region (SALK_027326). We identified the homozygous plants from a segregating parental line by PCR. The corresponding PCR products were identical to those of a confirmed homozygous line (SALK_027326C). However, the ABA1 transcript abundance in isolated homozygous line was almost identical to the ABA1 transcript abundance in the wild-type plants, as was revealed by RT-qPCR analysis. Because the phenotype of the selected homozygous line was also similar to wildtype plants in response to a low light, we conclude that the 5&apos; UTR mutation in the ABA1 gene does not affect the gene function and thus this line is not an eligible candidate for further genetic studies.

Název v anglickém jazyce

Isolation and detail characterization of aba1 T-DNA insertion mutant line of Arabidopsis thaliana

Popis výsledku anglicky

Abscisic acid is a plant hormone that affects many biological processes during plant lifespan including stomatal movement, seed and bud dormancy and organ size. Nevertheless, the role of abscisic acid in photomorphogenesis remains rudimentary. To study the role of abscisic acid in different light conditions, the characterization of homozygous mutant lines in biosynthetic gene encoding zeaxanthin epoxidase (ABA1) is essential for genetic studies. Here, we present isolation and characterization of one of the T-DNA insertion mutant lines of Arabidopsis thaliana ecotype Colombia-0, where the insertion is located in 5&apos; UTR region (SALK_027326). We identified the homozygous plants from a segregating parental line by PCR. The corresponding PCR products were identical to those of a confirmed homozygous line (SALK_027326C). However, the ABA1 transcript abundance in isolated homozygous line was almost identical to the ABA1 transcript abundance in the wild-type plants, as was revealed by RT-qPCR analysis. Because the phenotype of the selected homozygous line was also similar to wildtype plants in response to a low light, we conclude that the 5&apos; UTR mutation in the ABA1 gene does not affect the gene function and thus this line is not an eligible candidate for further genetic studies.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2018: Proceedings of International PhD Students Conference

ISBN

978-80-7509-597-8

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

5

Strana od-do

343-347

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

7. 11. 2018

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000462205300064