Isolation and detection of bacteria using magnetic molecularly imprinted polymers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43914708" target="_blank" >RIV/62156489:43210/18:43914708 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isolation and detection of bacteria using magnetic molecularly imprinted polymers

Popis výsledku v původním jazyce

A range of biological macromolecules as antibodies, enzymes or receptors function on principles based on selective recognition. These biological macromolecules are very often used in many technical applications due to their well-defined selectivity features. Unfortunately using of biomolecules is limited by their high production costs and low stability. Therefore, an idea of creation of synthetic materials with tailor-made molecular selectivity was presented. These materials are called molecularly imprinted polymers (MIPs). MIPs use molecular imprinting of chosen molecule to creating selective binding sites in cross-linked polymer. Technique of molecular imprinting has become one of the most efficient methods that are used for preparation of selective recognition materials. MIPs are stable, robust and have low production costs. In this work, the novel sensitive method for Staphylococcus aureus isolation and detection based on molecular imprinting was investigated. Molecularly imprinted layer was created on a surface of magnetic particles (MPs) due to ability of MPs pre-concentrate bacteria from large sample volumes. Fluorescence microscopy was used for detection of isolated bacteria.

Název v anglickém jazyce

Isolation and detection of bacteria using magnetic molecularly imprinted polymers

Popis výsledku anglicky

A range of biological macromolecules as antibodies, enzymes or receptors function on principles based on selective recognition. These biological macromolecules are very often used in many technical applications due to their well-defined selectivity features. Unfortunately using of biomolecules is limited by their high production costs and low stability. Therefore, an idea of creation of synthetic materials with tailor-made molecular selectivity was presented. These materials are called molecularly imprinted polymers (MIPs). MIPs use molecular imprinting of chosen molecule to creating selective binding sites in cross-linked polymer. Technique of molecular imprinting has become one of the most efficient methods that are used for preparation of selective recognition materials. MIPs are stable, robust and have low production costs. In this work, the novel sensitive method for Staphylococcus aureus isolation and detection based on molecular imprinting was investigated. Molecularly imprinted layer was created on a surface of magnetic particles (MPs) due to ability of MPs pre-concentrate bacteria from large sample volumes. Fluorescence microscopy was used for detection of isolated bacteria.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10401 - Organic chemistry

Projekt

<a href="/cs/project/GJ16-23647Y" target="_blank" >GJ16-23647Y: Zlepšení vlastností kapilární elektroforézy pomocí nanomateriálů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2018: Proceedings of International PhD Students Conference

ISBN

978-80-7509-597-8

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

5

Strana od-do

484-488

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

7. 11. 2018

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000462205300092