Complex cytotoxicity mechanism of bundles formed from self-organised 1-D anodic TiO2 nanotubes layers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43917433" target="_blank" >RIV/62156489:43210/20:43917433 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216275:25310/20:39916344 RIV/00216305:26620/20:PU136535

Výsledek na webu

<a href="https://doi.org/10.1016/j.jhazmat.2020.122054" target="_blank" >https://doi.org/10.1016/j.jhazmat.2020.122054</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jhazmat.2020.122054" target="_blank" >10.1016/j.jhazmat.2020.122054</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Complex cytotoxicity mechanism of bundles formed from self-organised 1-D anodic TiO2 nanotubes layers

Popis výsledku v původním jazyce

The present study reports on a comprehensive investigation of mechanisms of in vitro cytotoxicity of high aspect ratio (HAR) bundles formed from anodic TiO2 nanotube (TNT) layers. Comparative cytotoxicity studies were performed using two types of HAR TNTs (diameter of TILDE OPERATOR+D91110 nm), differing in initial thickness of the nanotubular layer (TILDE OPERATOR+D9135 μm for TNTs-1 vs. TILDE OPERATOR+D9110 μm for TNTs-2). Using two types of epithelial cell lines (MDA-MB-231, HEK-293), it was found that nanotoxicity is highly cell-type dependent and plausibly associates with higher membrane fluidity and decreased rigidity of cancer cells enabling penetration of TNTs to the cell membrane towards disruption of membrane integrity and reorganization of cytoskeletal network. Upon penetration, TNTs dysregulated redox homeostasis followed by DNA fragmentation and apoptotic/necrotic cell death. Both TNTs exhibited haemolytic activity and rapidly activated polarization of RAW 264.7 macrophages. Throughout the whole study, TNTs-2 possessing a lower aspect ratio manifested significantly higher cytotoxic effects. Taken together, this is the first report comprehensively investigating the mechanisms underlying the nanotoxicity of bundles formed from self-organised 1-D anodic TNT layers. Except for description of nanotoxicity of industrially-interesting nanomaterials, the delineation of the nanotoxicity paradigm in cancer cells could serve as solid basis for future efforts in rational engineering of TNTs towards selective anticancer nanomedicine.

Název v anglickém jazyce

Complex cytotoxicity mechanism of bundles formed from self-organised 1-D anodic TiO2 nanotubes layers

Popis výsledku anglicky

The present study reports on a comprehensive investigation of mechanisms of in vitro cytotoxicity of high aspect ratio (HAR) bundles formed from anodic TiO2 nanotube (TNT) layers. Comparative cytotoxicity studies were performed using two types of HAR TNTs (diameter of TILDE OPERATOR+D91110 nm), differing in initial thickness of the nanotubular layer (TILDE OPERATOR+D9135 μm for TNTs-1 vs. TILDE OPERATOR+D9110 μm for TNTs-2). Using two types of epithelial cell lines (MDA-MB-231, HEK-293), it was found that nanotoxicity is highly cell-type dependent and plausibly associates with higher membrane fluidity and decreased rigidity of cancer cells enabling penetration of TNTs to the cell membrane towards disruption of membrane integrity and reorganization of cytoskeletal network. Upon penetration, TNTs dysregulated redox homeostasis followed by DNA fragmentation and apoptotic/necrotic cell death. Both TNTs exhibited haemolytic activity and rapidly activated polarization of RAW 264.7 macrophages. Throughout the whole study, TNTs-2 possessing a lower aspect ratio manifested significantly higher cytotoxic effects. Taken together, this is the first report comprehensively investigating the mechanisms underlying the nanotoxicity of bundles formed from self-organised 1-D anodic TNT layers. Except for description of nanotoxicity of industrially-interesting nanomaterials, the delineation of the nanotoxicity paradigm in cancer cells could serve as solid basis for future efforts in rational engineering of TNTs towards selective anticancer nanomedicine.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30108 - Toxicology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Hazardous Materials

ISSN

0304-3894

e-ISSN

—

Svazek periodika

388

Číslo periodika v rámci svazku

15 April

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

122054

Kód UT WoS článku

000524462500109

EID výsledku v databázi Scopus

2-s2.0-85077754730