Novel method for morphometry analysis of bursa of Fabricius

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43919512" target="_blank" >RIV/62156489:43210/20:43919512 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Novel method for morphometry analysis of bursa of Fabricius

Popis výsledku v původním jazyce

Morphological observation of bursa of Fabricius (BF) represent a valuable tool in evaluation of stress causative agents on poultry. The main goal of this study was to compare two methods for morphometry analysis of BF: standard hematoxylin/eosin (HE/EO) staining and immunofluorescence staining based on anti-basal cell cytokeratin antibody with DAPI background staining (CYT/DAPI). Chickens were divided into two groups (18 for HE/EO and 18 for CYT/DAPI). BF from 6 chickens from each group were taken in 7, 14 and 22 days of age. In every chicken, 40 randomly selected follicles were evaluated. In total, comparison of data from all days between groups didn&apos;t show significant differences. However, age have a significant impact. In 14 days of age, size of the follicle and cortex were significantly higher in CYT/DAPI group (P&lt;0.05). Additionally, in 22 days old chickens size of the medulla was significantly higher in HE/EO group. Cortex/medulla ratio at age 7 days was significantly higher in HE/EO (P&lt;0.05), but in older chickens were significantly higher (P&lt;0.05) in CYT/DAPI group. Immunofluorescence staining seems to be more exact method and should be used, if precise localization of cortico-medullary border is necessary. On the other hand, HE/EO staining is a better choice for quantitative analyses of higher number of samples.

Název v anglickém jazyce

Novel method for morphometry analysis of bursa of Fabricius

Popis výsledku anglicky

Morphological observation of bursa of Fabricius (BF) represent a valuable tool in evaluation of stress causative agents on poultry. The main goal of this study was to compare two methods for morphometry analysis of BF: standard hematoxylin/eosin (HE/EO) staining and immunofluorescence staining based on anti-basal cell cytokeratin antibody with DAPI background staining (CYT/DAPI). Chickens were divided into two groups (18 for HE/EO and 18 for CYT/DAPI). BF from 6 chickens from each group were taken in 7, 14 and 22 days of age. In every chicken, 40 randomly selected follicles were evaluated. In total, comparison of data from all days between groups didn&apos;t show significant differences. However, age have a significant impact. In 14 days of age, size of the follicle and cortex were significantly higher in CYT/DAPI group (P&lt;0.05). Additionally, in 22 days old chickens size of the medulla was significantly higher in HE/EO group. Cortex/medulla ratio at age 7 days was significantly higher in HE/EO (P&lt;0.05), but in older chickens were significantly higher (P&lt;0.05) in CYT/DAPI group. Immunofluorescence staining seems to be more exact method and should be used, if precise localization of cortico-medullary border is necessary. On the other hand, HE/EO staining is a better choice for quantitative analyses of higher number of samples.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2020: Proceedings of International PhD Students Conference

ISBN

978-80-7509-765-1

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

439-443

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

11. 11. 2020

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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