Genetic analysis of selected genes active in chickpea seed dormancy and germination testing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43919581" target="_blank" >RIV/62156489:43210/20:43919581 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genetic analysis of selected genes active in chickpea seed dormancy and germination testing

Popis výsledku v původním jazyce

The most important event in the plant life cycle is transition from seed to seedling. Germination is an irreversible process and therefore correct timing is required. Several dormancy mechanisms exist among plants. Physical dormancy can be observed in legumes. Recombinant inbred lines derived from crossing of cultivated and wild parents of chickpea serve as a good model to clarify seed dormancy loss during crop domestication and its genetic basis. Restriction analysis was carried out using the cleaving of amplified polymorphic sequences method. For 6 candidate loci located on intervals of chromosome 1 and 5 were determined possible primer combinations with products that showed variability over non-coding regions. Polymorphic restriction recognition sites were found and restriction mapping was done with parental genotypes. Testing of optimal conditions for restriction analysis and primer combinations for population mapping are still ongoing process. In addition, the presence of physical dormancy in seeds was observed during germination testing. Two parental genotypes and population of 51 inbred lines were evaluated over 7 days&apos; period. Final germination percentage ranged from 20-100% and divided population into dormant and non-dormant group.

Název v anglickém jazyce

Genetic analysis of selected genes active in chickpea seed dormancy and germination testing

Popis výsledku anglicky

The most important event in the plant life cycle is transition from seed to seedling. Germination is an irreversible process and therefore correct timing is required. Several dormancy mechanisms exist among plants. Physical dormancy can be observed in legumes. Recombinant inbred lines derived from crossing of cultivated and wild parents of chickpea serve as a good model to clarify seed dormancy loss during crop domestication and its genetic basis. Restriction analysis was carried out using the cleaving of amplified polymorphic sequences method. For 6 candidate loci located on intervals of chromosome 1 and 5 were determined possible primer combinations with products that showed variability over non-coding regions. Polymorphic restriction recognition sites were found and restriction mapping was done with parental genotypes. Testing of optimal conditions for restriction analysis and primer combinations for population mapping are still ongoing process. In addition, the presence of physical dormancy in seeds was observed during germination testing. Two parental genotypes and population of 51 inbred lines were evaluated over 7 days&apos; period. Final germination percentage ranged from 20-100% and divided population into dormant and non-dormant group.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10611 - Plant sciences, botany

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2020: Proceedings of International PhD Students Conference

ISBN

978-80-7509-765-1

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

390-395

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

11. 11. 2020

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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