Bull sperm capacitation treatment and oocyte-sperm co-culture system affect in vitro bovine embryo development

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43919587" target="_blank" >RIV/62156489:43210/20:43919587 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Bull sperm capacitation treatment and oocyte-sperm co-culture system affect in vitro bovine embryo development

Popis výsledku v původním jazyce

The study was designed to define the effects of bull sperm treatment and co-culture system on in vitro embryo development. The mature oocytes were fertilized by sperm treated with heparin in standard co-culture (Exp.1) or by sperm treated with heparin supplemented either with PHE mix (penicillamine, hypotaurine, and epinephrine) or caffeine both in standard co-culture and in drops (Exp.2). A variability in the blastocyst rate was observed among bulls after fertilization of oocytes with heparin treated-sperm in standard co-culture. The significantly higher percentages of cleaved oocytes and developed blastocysts were derived from sperm treated with heparin-PHE in comparison with those treated with heparin-caffeine in drops (84.6 and 43.3 vs. 67.6 and 24.8, respectively). The significantly higher percentages of cleaved oocytes and developed blastocysts were recovered from sperm treated with heparin-PHE in drops than from those treated with heparin-caffeine in standard co-culture (85.9 and 47.1 vs. 73.3 and 25.6, respectively). We concluded, that the efficiency of in vitro bovine embryo development depends on sperm capacitation treatment and oocyte-sperm co-culture system.

Název v anglickém jazyce

Bull sperm capacitation treatment and oocyte-sperm co-culture system affect in vitro bovine embryo development

Popis výsledku anglicky

The study was designed to define the effects of bull sperm treatment and co-culture system on in vitro embryo development. The mature oocytes were fertilized by sperm treated with heparin in standard co-culture (Exp.1) or by sperm treated with heparin supplemented either with PHE mix (penicillamine, hypotaurine, and epinephrine) or caffeine both in standard co-culture and in drops (Exp.2). A variability in the blastocyst rate was observed among bulls after fertilization of oocytes with heparin treated-sperm in standard co-culture. The significantly higher percentages of cleaved oocytes and developed blastocysts were derived from sperm treated with heparin-PHE in comparison with those treated with heparin-caffeine in drops (84.6 and 43.3 vs. 67.6 and 24.8, respectively). The significantly higher percentages of cleaved oocytes and developed blastocysts were recovered from sperm treated with heparin-PHE in drops than from those treated with heparin-caffeine in standard co-culture (85.9 and 47.1 vs. 73.3 and 25.6, respectively). We concluded, that the efficiency of in vitro bovine embryo development depends on sperm capacitation treatment and oocyte-sperm co-culture system.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2020: Proceedings of International PhD Students Conference

ISBN

978-80-7509-765-1

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

430-434

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

11. 11. 2020

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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