Optimized assembly of functionalized-neuroblastoma targeting EcLHFRT nanocarriers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43919628" target="_blank" >RIV/62156489:43210/20:43919628 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Optimized assembly of functionalized-neuroblastoma targeting EcLHFRT nanocarriers

Popis výsledku v původním jazyce

Biomimetic peptide ligands designed by using computational biology and in silico chemistry can bring crucial results related to their possible application in targeted anticancer therapy, more specifically in the field of active targeting of malignant neuroblastoma cell lines by modified apoferritin nanocarriers (EcLHFRT) with encapsulated cytotoxic drug. Using the high-performance throughput computing technology, unique peptides (CpTrkA, CpNGF, CpNT4) were designed and synthesized in this work. These peptides showed high affinity in silico to tropomyosin receptor kinases (TrkA, TrkB, TrkC), which are expressed on membranes of neuroblastoma cells. The main aim of this work was detailed characterization of prepared EcLHFRT nanocarriers through which we evaluated the size, stability and hemolytic properties of EcLHFRT with unmodified or modified surface insomuch as mentioned parameters are key for the further research in neuroblastoma nanomedicine. The prepared EcLHFRT nanocarriers possessed optimal size (&lt;50 nm) and stability (ζ~ -20 mV) and in contrast to free cytotoxic drug, they did not cause any hemolysis.

Název v anglickém jazyce

Optimized assembly of functionalized-neuroblastoma targeting EcLHFRT nanocarriers

Popis výsledku anglicky

Biomimetic peptide ligands designed by using computational biology and in silico chemistry can bring crucial results related to their possible application in targeted anticancer therapy, more specifically in the field of active targeting of malignant neuroblastoma cell lines by modified apoferritin nanocarriers (EcLHFRT) with encapsulated cytotoxic drug. Using the high-performance throughput computing technology, unique peptides (CpTrkA, CpNGF, CpNT4) were designed and synthesized in this work. These peptides showed high affinity in silico to tropomyosin receptor kinases (TrkA, TrkB, TrkC), which are expressed on membranes of neuroblastoma cells. The main aim of this work was detailed characterization of prepared EcLHFRT nanocarriers through which we evaluated the size, stability and hemolytic properties of EcLHFRT with unmodified or modified surface insomuch as mentioned parameters are key for the further research in neuroblastoma nanomedicine. The prepared EcLHFRT nanocarriers possessed optimal size (&lt;50 nm) and stability (ζ~ -20 mV) and in contrast to free cytotoxic drug, they did not cause any hemolysis.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA17-12816S" target="_blank" >GA17-12816S: Konstrukce modifikovaných apoferitinových nanočástic s protinádorovými léčivy a studium mechanismů potencujících jejich efektivitu v terapii</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2020: Proceedings of International PhD Students Conference

ISBN

978-80-7509-765-1

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

603-608

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

11. 11. 2020

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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