Silver nanoparticles eliminate Xanthomonas campestris pv. campestris in cabbage seeds more efficiently than hot water treatment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43919685" target="_blank" >RIV/62156489:43210/21:43919685 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43510/21:43919685 RIV/00216305:26620/21:PU141397 RIV/00216224:14740/21:00124440

Výsledek na webu

<a href="https://doi.org/10.1016/j.mtcomm.2021.102284" target="_blank" >https://doi.org/10.1016/j.mtcomm.2021.102284</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mtcomm.2021.102284" target="_blank" >10.1016/j.mtcomm.2021.102284</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Silver nanoparticles eliminate Xanthomonas campestris pv. campestris in cabbage seeds more efficiently than hot water treatment

Popis výsledku v původním jazyce

Silver nanoparticles (AgNPs) and the standard hot water treatment (HWT) method were examined as measures against the bacterium Xanthomonas campestris pv. campestris (Xcc). Three sizes (TILDE OPERATOR+D912 nm, TILDE OPERATOR+D9122 nm and TILDE OPERATOR+D9129 nm) of AgNPs were synthesized and characterized using transmission electron microscopy and dynamic light scattering. To evaluate the direct effect of the synthesized AgNPs on Xcc, an in vitro colony counting assay was carried out. Cabbage seeds (cv.&apos; Avak F1&apos;) were inoculated with an Xcc (strain 1279a) suspension and treated with hot water or a AgNP solution. The Xcc infestation in the young seedlings was quantified using real-time PCR and dilution plating assays. The results of the colony counting assay indicated that the antibacterial effect of the AgNPs depended on the size and concentration of the NPs. The seedling analysis showed that all three sizes of AgNPs suppressed Xcc infestation at concentrations of 6.25 μg mLMINUS SIGN 1 and higher. HWT also had an antibacterial effect; nevertheless, in contrast to AgNP treatment, HWT strongly decreased the germination rate of treated seeds.

Název v anglickém jazyce

Silver nanoparticles eliminate Xanthomonas campestris pv. campestris in cabbage seeds more efficiently than hot water treatment

Popis výsledku anglicky

Silver nanoparticles (AgNPs) and the standard hot water treatment (HWT) method were examined as measures against the bacterium Xanthomonas campestris pv. campestris (Xcc). Three sizes (TILDE OPERATOR+D912 nm, TILDE OPERATOR+D9122 nm and TILDE OPERATOR+D9129 nm) of AgNPs were synthesized and characterized using transmission electron microscopy and dynamic light scattering. To evaluate the direct effect of the synthesized AgNPs on Xcc, an in vitro colony counting assay was carried out. Cabbage seeds (cv.&apos; Avak F1&apos;) were inoculated with an Xcc (strain 1279a) suspension and treated with hot water or a AgNP solution. The Xcc infestation in the young seedlings was quantified using real-time PCR and dilution plating assays. The results of the colony counting assay indicated that the antibacterial effect of the AgNPs depended on the size and concentration of the NPs. The seedling analysis showed that all three sizes of AgNPs suppressed Xcc infestation at concentrations of 6.25 μg mLMINUS SIGN 1 and higher. HWT also had an antibacterial effect; nevertheless, in contrast to AgNP treatment, HWT strongly decreased the germination rate of treated seeds.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials Today Communications

ISSN

2352-4928

e-ISSN

—

Svazek periodika

27

Číslo periodika v rámci svazku

June

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

102284

Kód UT WoS článku

000683031900009

EID výsledku v databázi Scopus

2-s2.0-85103696384