Molecularly imprinted polymer nanoparticles as potential tool for immunohistochemistry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43919820" target="_blank" >RIV/62156489:43210/21:43919820 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.37904/nanocon.2020.3751" target="_blank" >https://doi.org/10.37904/nanocon.2020.3751</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2020.3751" target="_blank" >10.37904/nanocon.2020.3751</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecularly imprinted polymer nanoparticles as potential tool for immunohistochemistry

Popis výsledku v původním jazyce

Immunohistochemistry (IHC) is a powerful technique enabling visualization/detection of cellular components (e.g. overexpressed proteins) in tissue samples and thus ensuring timely recognition of a wide range of diseases. Nowadays, antibodies labelled by different kinds of sensing probes are routinely used for selective recognition of IHC markers. However, antibodies as bio-macromolecules have a number of drawbacks including physical and (bio) chemical instability or large production cost. Therefore, the design and synthesis of artificial antibodies that are able to bind a target molecule with similar affinity and specificity have become a long-term goal in a range of scientific branches. In this work, molecularly imprinted polymer nanoparticles (MIPs), as antibody mimics, were used as IHC agents. Specifically, polydopamine MIP nanoparticles doped with metal ions and selective for metallothionein as potential IHC marker were developed and combined with laser ablation ICP-MS. This novel approach can significantly enhance the abilities of IHC analysis and solve issues connected with the low stability of antibodies.

Název v anglickém jazyce

Molecularly imprinted polymer nanoparticles as potential tool for immunohistochemistry

Popis výsledku anglicky

Immunohistochemistry (IHC) is a powerful technique enabling visualization/detection of cellular components (e.g. overexpressed proteins) in tissue samples and thus ensuring timely recognition of a wide range of diseases. Nowadays, antibodies labelled by different kinds of sensing probes are routinely used for selective recognition of IHC markers. However, antibodies as bio-macromolecules have a number of drawbacks including physical and (bio) chemical instability or large production cost. Therefore, the design and synthesis of artificial antibodies that are able to bind a target molecule with similar affinity and specificity have become a long-term goal in a range of scientific branches. In this work, molecularly imprinted polymer nanoparticles (MIPs), as antibody mimics, were used as IHC agents. Specifically, polydopamine MIP nanoparticles doped with metal ions and selective for metallothionein as potential IHC marker were developed and combined with laser ablation ICP-MS. This novel approach can significantly enhance the abilities of IHC analysis and solve issues connected with the low stability of antibodies.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NANOCON 2020: Conference Proceedings

ISBN

978-80-87294-98-7

ISSN

2694-930X

e-ISSN

2694-930X

Počet stran výsledku

5

Strana od-do

330-334

Název nakladatele

Tanger Ltd.

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

21. 10. 2020

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000664505500056