Using QD-FRET based method to investigate protein-protein interactions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43919821" target="_blank" >RIV/62156489:43210/21:43919821 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/21:PU141192

Výsledek na webu

<a href="https://doi.org/10.37904/nanocon.2020.3750" target="_blank" >https://doi.org/10.37904/nanocon.2020.3750</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2020.3750" target="_blank" >10.37904/nanocon.2020.3750</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Using QD-FRET based method to investigate protein-protein interactions

Popis výsledku v původním jazyce

Förster resonance energy transfer (FRET) is an energy transfer process between a pair of light-sensitive molecules, where the donor fluorophore (initially in its electronic excited state) transfers energy to an acceptor chromophore. In this study, FRET was used to investigate the dimerization of metallothionein isoform MT-1A. The FRET system was developed based on a fluorescent quantum dots (QDs) and cyanine dye 3.5 (Cy3.5) as a versatile tool to probe small distance changes between acceptor and donor fluorophores in nanometer range. Herein, the water-soluble 450-nm emitting QDs as the donor and 590-nm Cy3.5 as the acceptor were covalently conjugated with MT-1 according to the protocol. Previous studies suggest that MTs may form oligomers under certain conditions. Therefore, further studies of this phenomenon, which has been studied here using capillary electrophoresis (CE) coupled with fluorescence detection.

Název v anglickém jazyce

Using QD-FRET based method to investigate protein-protein interactions

Popis výsledku anglicky

Förster resonance energy transfer (FRET) is an energy transfer process between a pair of light-sensitive molecules, where the donor fluorophore (initially in its electronic excited state) transfers energy to an acceptor chromophore. In this study, FRET was used to investigate the dimerization of metallothionein isoform MT-1A. The FRET system was developed based on a fluorescent quantum dots (QDs) and cyanine dye 3.5 (Cy3.5) as a versatile tool to probe small distance changes between acceptor and donor fluorophores in nanometer range. Herein, the water-soluble 450-nm emitting QDs as the donor and 590-nm Cy3.5 as the acceptor were covalently conjugated with MT-1 according to the protocol. Previous studies suggest that MTs may form oligomers under certain conditions. Therefore, further studies of this phenomenon, which has been studied here using capillary electrophoresis (CE) coupled with fluorescence detection.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NANOCON 2020: Conference Proceedings

ISBN

978-80-87294-98-7

ISSN

2694-930X

e-ISSN

2694-930X

Počet stran výsledku

5

Strana od-do

390-394

Název nakladatele

Tanger Ltd.

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

21. 10. 2020

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000664505500066