Ovarian steroid hormone secretion by human granulosa cells after supplementation of sambucus nigra L. extract

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43920631" target="_blank" >RIV/62156489:43210/21:43920631 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.33549/physiolres.934680" target="_blank" >https://doi.org/10.33549/physiolres.934680</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.33549/physiolres.934680" target="_blank" >10.33549/physiolres.934680</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ovarian steroid hormone secretion by human granulosa cells after supplementation of sambucus nigra L. extract

Popis výsledku v původním jazyce

Beneficial effects of Sambucus nigra L. (black elder) as a traditional medicine have been associated with the phytoconstituents including polyphenols, terpenes and lectins. Various antioxidant rich natural products have also been implicated with improvement of reproductive health and fertility, however, the effect of Sambucus nigra on the ovarian cell functions has not been investigated yet. The objectives of the present study were to screen the polyphenols in the elderflower and elderberry extracts, and to examine the secretion activity of steroid hormones 17β-estradiol and progesterone by human ovarian granulosa cells HGL5 after supplementation of the extracts at a concentration range of 12.5 to 100 μg.ml-1. Qualitative as well as quantitative screening of polyphenols by high-performance liquid chromatography with diode-array detector (HPLC-DAD) analysis revealed rutin to be the most abundant polyphenol in both elderflower and elderberry extracts. In culture, neither elderflower nor elderberry extract caused any significant impact (p&gt;0.05) in cell viability as studied by AlamarBlue assay in comparison to control. However, a dosedependent stimulation of 17β-estradiol release was detected by ELISA after supplementation of elderflower (at 50 μg.ml-1; p&lt;0.01) and elderberry (at 100 μg.ml-1; p&lt;0.05) extracts at higher doses used in the study. On the other hand, both elderflower and elderberry extracts stimulated the secretion of progesterone by HGL5 cells at a lower dose (12.5 μg.ml-1; p&lt;0.05), as compared to control. Therefore, elderflower and elderberry extracts may have the potential to regulate steroidogenesis in ovarian cells.

Název v anglickém jazyce

Ovarian steroid hormone secretion by human granulosa cells after supplementation of sambucus nigra L. extract

Popis výsledku anglicky

Beneficial effects of Sambucus nigra L. (black elder) as a traditional medicine have been associated with the phytoconstituents including polyphenols, terpenes and lectins. Various antioxidant rich natural products have also been implicated with improvement of reproductive health and fertility, however, the effect of Sambucus nigra on the ovarian cell functions has not been investigated yet. The objectives of the present study were to screen the polyphenols in the elderflower and elderberry extracts, and to examine the secretion activity of steroid hormones 17β-estradiol and progesterone by human ovarian granulosa cells HGL5 after supplementation of the extracts at a concentration range of 12.5 to 100 μg.ml-1. Qualitative as well as quantitative screening of polyphenols by high-performance liquid chromatography with diode-array detector (HPLC-DAD) analysis revealed rutin to be the most abundant polyphenol in both elderflower and elderberry extracts. In culture, neither elderflower nor elderberry extract caused any significant impact (p&gt;0.05) in cell viability as studied by AlamarBlue assay in comparison to control. However, a dosedependent stimulation of 17β-estradiol release was detected by ELISA after supplementation of elderflower (at 50 μg.ml-1; p&lt;0.01) and elderberry (at 100 μg.ml-1; p&lt;0.05) extracts at higher doses used in the study. On the other hand, both elderflower and elderberry extracts stimulated the secretion of progesterone by HGL5 cells at a lower dose (12.5 μg.ml-1; p&lt;0.05), as compared to control. Therefore, elderflower and elderberry extracts may have the potential to regulate steroidogenesis in ovarian cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30304 - Public and environmental health

Projekt

<a href="/cs/project/8X20023" target="_blank" >8X20023: Dopad trávicích a vstřebávacích procesů na konečnou biologickou aktivitu rostlinných fytonutrientů: skutečná přidaná hodnota pro zdraví</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Research

ISSN

0862-8408

e-ISSN

—

Svazek periodika

70

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

755-764

Kód UT WoS článku

000729810300009

EID výsledku v databázi Scopus

2-s2.0-85122334323