Exploring the pH-triggerable structure of siRNA-carrying liposomal nanoparticles as tools for treatment of hepatitis B

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43921644" target="_blank" >RIV/62156489:43210/21:43921644 - isvavai.cz</a>

Výsledek na webu

<a href="https://mendelnet.cz/media/mnet_2021_full.pdf" target="_blank" >https://mendelnet.cz/media/mnet_2021_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Exploring the pH-triggerable structure of siRNA-carrying liposomal nanoparticles as tools for treatment of hepatitis B

Popis výsledku v původním jazyce

Gene therapy using small interfering RNA (siRNA) molecules provides silencing of undesirable genes on mRNA translational level, which eliminates potential risks related to interactions with genomic DNA of the cell. However, due to their instability, these siRNA molecules must be delivered to the site of action via sophisticated delivery system. In this research work, we opted for liposomal vectors modified with polyethylene glycol chains attached by a pH-sensitive linkage. These liposomal nanoparticles were monitored in different buffer and pH conditions to verify some of their physicochemical properties required for an efficient siRNA delivery. Results from light scattering methods and cryo-transmission electron microscopy revealed that higher ionic strength environment reduced the diameter and zeta potential of the nanoparticles and that mildly acidic pH (6.0) caused structural changes leading to aggregation of the nanoparticles. These findings predetermine their favourable behaviour in biological systems.

Název v anglickém jazyce

Exploring the pH-triggerable structure of siRNA-carrying liposomal nanoparticles as tools for treatment of hepatitis B

Popis výsledku anglicky

Gene therapy using small interfering RNA (siRNA) molecules provides silencing of undesirable genes on mRNA translational level, which eliminates potential risks related to interactions with genomic DNA of the cell. However, due to their instability, these siRNA molecules must be delivered to the site of action via sophisticated delivery system. In this research work, we opted for liposomal vectors modified with polyethylene glycol chains attached by a pH-sensitive linkage. These liposomal nanoparticles were monitored in different buffer and pH conditions to verify some of their physicochemical properties required for an efficient siRNA delivery. Results from light scattering methods and cryo-transmission electron microscopy revealed that higher ionic strength environment reduced the diameter and zeta potential of the nanoparticles and that mildly acidic pH (6.0) caused structural changes leading to aggregation of the nanoparticles. These findings predetermine their favourable behaviour in biological systems.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2021: Proceedings of International PhD Students Conference

ISBN

978-80-7509-821-4

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

454-457

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

10. 11. 2021

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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