Method for simultaneous detection of Phytophthora infestans proteins and DNA in Solanum tuberosum samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43921815" target="_blank" >RIV/62156489:43210/21:43921815 - isvavai.cz</a>

Výsledek na webu

<a href="https://mendelnet.cz/media/mnet_2021_full.pdf" target="_blank" >https://mendelnet.cz/media/mnet_2021_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Method for simultaneous detection of Phytophthora infestans proteins and DNA in Solanum tuberosum samples

Popis výsledku v původním jazyce

Phytophthora infestans is a model organism for the oomycetes, a distinct lineage of funguslike eukaryotes. However, P. infestans is primarily known as a pathogen that causes the serious potato and tomato disease known as late blight. The means of controlling late blight are limited and require early detection of the pathogen. One of the most reliable method for targeted quantitation is based on qPCR. The amount of sample is often limited, which does not allow the use of other analyses such as proteome analysis by LC-MS. Here, we describe the optimized extraction method for simultaneously extracting the DNA and proteins from an infected leaf of Solanum tuberosum. Simultaneous analysis of the amount of P. infestans DNA and the proteome composition from the infected leaf opens possibilities for a better understanding of the interaction between P. infestans and the plant.

Název v anglickém jazyce

Method for simultaneous detection of Phytophthora infestans proteins and DNA in Solanum tuberosum samples

Popis výsledku anglicky

Phytophthora infestans is a model organism for the oomycetes, a distinct lineage of funguslike eukaryotes. However, P. infestans is primarily known as a pathogen that causes the serious potato and tomato disease known as late blight. The means of controlling late blight are limited and require early detection of the pathogen. One of the most reliable method for targeted quantitation is based on qPCR. The amount of sample is often limited, which does not allow the use of other analyses such as proteome analysis by LC-MS. Here, we describe the optimized extraction method for simultaneously extracting the DNA and proteins from an infected leaf of Solanum tuberosum. Simultaneous analysis of the amount of P. infestans DNA and the proteome composition from the infected leaf opens possibilities for a better understanding of the interaction between P. infestans and the plant.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK1910045" target="_blank" >QK1910045: Identifikace metabolitů korelujících s kvantitativní rezistencí k Phytophthora infestans</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2021: Proceedings of International PhD Students Conference

ISBN

978-80-7509-821-4

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

337-341

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

10. 11. 2021

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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