Cross-linked-Pd0 polyethyleneimine catalyst for bioorthogonal chemistry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43921841" target="_blank" >RIV/62156489:43210/21:43921841 - isvavai.cz</a>

Výsledek na webu

<a href="https://mendelnet.cz/media/mnet_2021_full.pdf" target="_blank" >https://mendelnet.cz/media/mnet_2021_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cross-linked-Pd0 polyethyleneimine catalyst for bioorthogonal chemistry

Popis výsledku v původním jazyce

Bioorthogonal therapy represents a promising tool for controlled pro-drug activation. However, efficient catalysis in the intracellular environment mediated by organometallic compounds still poses a great challenge. Herein, we describe the preparation of a palladium-trapped cross-linked catalyst. Polycationic polymer polyethyleneimine (PEI), an efficient transfection agent, was used as a carrier for palladium catalyst. To prevent palladium leaching and undesired palladium inactivation, PEI polymer with nested palladium catalyst was cross-linked with phosphine compound tetrakis(hydroxymethyl)phosphine chloride (THPC). The catalytic activity of cross-linked-Pd-PEI catalyst was assessed by the activation of a precursor fluorescent probe. The prepared catalyst exhibited catalytic activity in cell-free conditions in a PBS environment, as well as in a cell culture medium supplemented with FBS, which simulated the cellular environment.

Název v anglickém jazyce

Cross-linked-Pd0 polyethyleneimine catalyst for bioorthogonal chemistry

Popis výsledku anglicky

Bioorthogonal therapy represents a promising tool for controlled pro-drug activation. However, efficient catalysis in the intracellular environment mediated by organometallic compounds still poses a great challenge. Herein, we describe the preparation of a palladium-trapped cross-linked catalyst. Polycationic polymer polyethyleneimine (PEI), an efficient transfection agent, was used as a carrier for palladium catalyst. To prevent palladium leaching and undesired palladium inactivation, PEI polymer with nested palladium catalyst was cross-linked with phosphine compound tetrakis(hydroxymethyl)phosphine chloride (THPC). The catalytic activity of cross-linked-Pd-PEI catalyst was assessed by the activation of a precursor fluorescent probe. The prepared catalyst exhibited catalytic activity in cell-free conditions in a PBS environment, as well as in a cell culture medium supplemented with FBS, which simulated the cellular environment.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2021: Proceedings of International PhD Students Conference

ISBN

978-80-7509-821-4

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

469-474

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

10. 11. 2021

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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