Bunyaviruses Affect Growth, Sporulation, and Elicitin Production in Phytophthora cactorum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F22%3A43922811" target="_blank" >RIV/62156489:43210/22:43922811 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43410/22:43922811

Výsledek na webu

<a href="https://doi.org/10.3390/v14122596" target="_blank" >https://doi.org/10.3390/v14122596</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/v14122596" target="_blank" >10.3390/v14122596</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bunyaviruses Affect Growth, Sporulation, and Elicitin Production in Phytophthora cactorum

Popis výsledku v původním jazyce

Phytophthora cactorum is an important oomycetous plant pathogen with numerous host plant species, including garden strawberry (Fragaria x ananassa) and silver birch (Betula pendula). P. cactorum also hosts mycoviruses, but their phenotypic effects on the host oomycete have not been studied earlier. In the present study, we tested polyethylene glycol (PEG)-induced water stress for virus curing and created an isogenic virus-free isolate for testing viral effects in pair with the original isolate. Phytophthora cactorum bunya-like viruses 1 and 2 (PcBV1 &amp; 2) significantly reduced hyphal growth of the P. cactorum host isolate, as well as sporangia production and size. Transcriptomic and proteomic analyses revealed an increase in the production of elicitins due to bunyavirus infection. However, the presence of bunyaviruses did not seem to alter the pathogenicity of P. cactorum. Virus transmission through anastomosis was unsuccessful in vitro.

Název v anglickém jazyce

Bunyaviruses Affect Growth, Sporulation, and Elicitin Production in Phytophthora cactorum

Popis výsledku anglicky

Phytophthora cactorum is an important oomycetous plant pathogen with numerous host plant species, including garden strawberry (Fragaria x ananassa) and silver birch (Betula pendula). P. cactorum also hosts mycoviruses, but their phenotypic effects on the host oomycete have not been studied earlier. In the present study, we tested polyethylene glycol (PEG)-induced water stress for virus curing and created an isogenic virus-free isolate for testing viral effects in pair with the original isolate. Phytophthora cactorum bunya-like viruses 1 and 2 (PcBV1 &amp; 2) significantly reduced hyphal growth of the P. cactorum host isolate, as well as sporangia production and size. Transcriptomic and proteomic analyses revealed an increase in the production of elicitins due to bunyavirus infection. However, the presence of bunyaviruses did not seem to alter the pathogenicity of P. cactorum. Virus transmission through anastomosis was unsuccessful in vitro.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40102 - Forestry

Projekt

<a href="/cs/project/EF15_003%2F0000453" target="_blank" >EF15_003/0000453: Výzkumné centrum pro studium patogenů z rodu Phytophthora</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Viruses

ISSN

1999-4915

e-ISSN

1999-4915

Svazek periodika

14

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

22

Strana od-do

2596

Kód UT WoS článku

000904320800001

EID výsledku v databázi Scopus

2-s2.0-85144495812