Particle Number of Aerosol in Specific Conditions of Biotechnology Laboratory Cleanroom

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F23%3A43923437" target="_blank" >RIV/62156489:43210/23:43923437 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.3390/app13105915" target="_blank" >https://doi.org/10.3390/app13105915</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/app13105915" target="_blank" >10.3390/app13105915</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Particle Number of Aerosol in Specific Conditions of Biotechnology Laboratory Cleanroom

Popis výsledku v původním jazyce

This paper presents the results of experimental measurements of the number of aerosol particles in cleanroom air of a biotechnology laboratory for DNA analysis. The aerosol content in the air can harm the accuracy of any analysis performed. The experimental measurements demonstrate compliance with the ISO cleanroom requirements for class 7. The indoor environment of the investigated biotechnology laboratory cleanroom can be classified as ISO cleanroom class 8. The particle count values depend on the month in which the measurement is made. The location of the measurement site, on the other hand, does not have too much influence. Our results showed that the values of particle number in the laboratory ranged from 325,965 to 2,740,877 pc/m3 for particles of 0.5 μm and more. From these measurements, it can be concluded that the biotechnology laboratory premises were not very suitable locations to perform for the intended DNA tests since microbiological contamination had the capacity to distort the results of these tests. As such, the authors propose several strategies to improve the current situation. These strategies are based on changing the laboratory work organization and cleaning methodology. In addition, the monitoring of particles with a size of 5 µm or more is recommended.

Název v anglickém jazyce

Particle Number of Aerosol in Specific Conditions of Biotechnology Laboratory Cleanroom

Popis výsledku anglicky

This paper presents the results of experimental measurements of the number of aerosol particles in cleanroom air of a biotechnology laboratory for DNA analysis. The aerosol content in the air can harm the accuracy of any analysis performed. The experimental measurements demonstrate compliance with the ISO cleanroom requirements for class 7. The indoor environment of the investigated biotechnology laboratory cleanroom can be classified as ISO cleanroom class 8. The particle count values depend on the month in which the measurement is made. The location of the measurement site, on the other hand, does not have too much influence. Our results showed that the values of particle number in the laboratory ranged from 325,965 to 2,740,877 pc/m3 for particles of 0.5 μm and more. From these measurements, it can be concluded that the biotechnology laboratory premises were not very suitable locations to perform for the intended DNA tests since microbiological contamination had the capacity to distort the results of these tests. As such, the authors propose several strategies to improve the current situation. These strategies are based on changing the laboratory work organization and cleaning methodology. In addition, the monitoring of particles with a size of 5 µm or more is recommended.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20701 - Environmental and geological engineering, geotechnics

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Sciences

ISSN

2076-3417

e-ISSN

2076-3417

Svazek periodika

13

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

12

Strana od-do

5915

Kód UT WoS článku

000995601300001

EID výsledku v databázi Scopus

2-s2.0-85160859514