Stanovení půdní proteázové aktivity při nízkých teplotách, nahrazení pufrovacího roztoku demineralizovanou vodou a po přídavku kaseinu

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F08%3A00128082" target="_blank" >RIV/62156489:43410/08:00128082 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67179843:_____/08:00341974

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Estimation of protease activity in soils at low temperatures by casein amendment and with substitution of buffer by demineralized water

Popis výsledku v původním jazyce

The aim of this work was to modify the method of Ladd and Buttler (1972), by substituting Tris--HCl buffer (pH 8.52) with demineralized water (DEMI H2O), in order to assess its suitability for measurement of casein-protease activity at pH levels close tothose of real soil in H2O. Measurements were undertaken over a range of incubation temperatures from 3 to 49 _C. Testing was performed on one organic soil and two different mineral soils. The substitution of Tris--HCl buffer by DEMI H2O at 49 _C decreased casein-protease activity to 67.25% in mineral soil and to 53.76% in organic soil. With decreasing temperature casein-protease activity decreased the most in organic soil, i.e., 0.07% of original its value at 3 _C. The incubation period was extended tomaximally 336 h at 3 _C to totally obtain >10.0% of L-tyrosine equivalents released at optimum or close to optimum temperature and pH conditions. The Q10 values of casein-protease activity measured after substituting Tris--HCl buffer wit

Název v anglickém jazyce

Estimation of protease activity in soils at low temperatures by casein amendment and with substitution of buffer by demineralized water

Popis výsledku anglicky

The aim of this work was to modify the method of Ladd and Buttler (1972), by substituting Tris--HCl buffer (pH 8.52) with demineralized water (DEMI H2O), in order to assess its suitability for measurement of casein-protease activity at pH levels close tothose of real soil in H2O. Measurements were undertaken over a range of incubation temperatures from 3 to 49 _C. Testing was performed on one organic soil and two different mineral soils. The substitution of Tris--HCl buffer by DEMI H2O at 49 _C decreased casein-protease activity to 67.25% in mineral soil and to 53.76% in organic soil. With decreasing temperature casein-protease activity decreased the most in organic soil, i.e., 0.07% of original its value at 3 _C. The incubation period was extended tomaximally 336 h at 3 _C to totally obtain >10.0% of L-tyrosine equivalents released at optimum or close to optimum temperature and pH conditions. The Q10 values of casein-protease activity measured after substituting Tris--HCl buffer wit

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

DF - Pedologie

OECD FORD obor

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Projekt

<a href="/cs/project/SM%2F640%2F18%2F03" target="_blank" >SM/640/18/03: CzechCarbo - studium cyklu uhlíku v terestrických ekosystémech ČR v souvislostech Evropského projektu CARBOEUROPE</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Amino Acids

ISSN

0939-4451

e-ISSN

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Svazek periodika

35

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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