Characterisation of czech Ophiostoma novo-ulmi isolates

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Characterisation of czech Ophiostoma novo-ulmi isolates

Popis výsledku v původním jazyce

Ophiostoma novo-ulmi was recorded for the first time in the area of the Czech Republic in 2006, although it was suspected since early 1960's. During the years 2006 and 2007 58 isolates were collected. Isolated strains of the DED causal agent were determined by PCR and RFLP molecular methods. DNA of each sample was isolated from cultured mycelium, amplified at cu, and col1 gene regions and restricted by Hph I and Bfa I endonucleases. Strains were determined according to both of these two gene regions. Anold species Ophiostoma ulmi (Buism.) Nannf. was not noticed in any case. Every sample belongs to the species Ophiostoma novo-ulmi Bras., and both known subspecies americana (5 isolates) and novo-ulmi (29 isolates) were present. Additionally, 13 strainsof Ophiostoma novo-ulmi were more particularly tested for vegetative compatibility type, mating type, fertility with both subspecies and cerato-ulmin production. Among these 13 strains only 3 seemed to be non-hybridized ssp. novo-ulmi and

Název v anglickém jazyce

Characterisation of czech Ophiostoma novo-ulmi isolates

Popis výsledku anglicky

Ophiostoma novo-ulmi was recorded for the first time in the area of the Czech Republic in 2006, although it was suspected since early 1960's. During the years 2006 and 2007 58 isolates were collected. Isolated strains of the DED causal agent were determined by PCR and RFLP molecular methods. DNA of each sample was isolated from cultured mycelium, amplified at cu, and col1 gene regions and restricted by Hph I and Bfa I endonucleases. Strains were determined according to both of these two gene regions. Anold species Ophiostoma ulmi (Buism.) Nannf. was not noticed in any case. Every sample belongs to the species Ophiostoma novo-ulmi Bras., and both known subspecies americana (5 isolates) and novo-ulmi (29 isolates) were present. Additionally, 13 strainsof Ophiostoma novo-ulmi were more particularly tested for vegetative compatibility type, mating type, fertility with both subspecies and cerato-ulmin production. Among these 13 strains only 3 seemed to be non-hybridized ssp. novo-ulmi and

Druh

D - Stať ve sborníku

CEP obor

GK - Lesnictví

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the conference of IUFRO working party 7.02.02 Egirdir, Turkey 11 - 16 May 2009

ISBN

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ISSN

1302-7085

e-ISSN

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Počet stran výsledku

5

Strana od-do

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Název nakladatele

Faculty of Forestry Journal Süleyman Demirel University

Místo vydání

Isparta

Místo konání akce

Egirdir, Turkey

Datum konání akce

1. 1. 2009

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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