Development of microsatellite and mating type markers for the pine needle pathogen Lecanosticta acicola

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F14%3A00213818" target="_blank" >RIV/62156489:43410/14:00213818 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/article/10.1007/s13313-013-0256-5" target="_blank" >http://link.springer.com/article/10.1007/s13313-013-0256-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13313-013-0256-5" target="_blank" >10.1007/s13313-013-0256-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of microsatellite and mating type markers for the pine needle pathogen Lecanosticta acicola

Popis výsledku v původním jazyce

Lecanosticta acicola is an ascomycete that causes brown spot needle blight of pine species in many regions of the world. This pathogen is responsible for a major disease of Pinus palustris in the USA and is a quarantine organism in Europe. In order to study the genetic diversity and patterns of spread of L. acicola, eleven microsatellite markers and two mating type markers were developed. An enrichment protocol was used to isolate microsatellite-rich DNA regions, and 18 primer pairs were designed to flank these regions, of which eleven were polymorphic. A total of 93 alleles were obtained across all loci from forty isolates of L. acicola from the USA with an allelic diversity range of 0.095 to 0.931 per locus. Cross-species amplification with some of the markers was obtained with L. gloeospora, L. guatemalensis and Dothistroma septosporum, but not with D. pini. Mating type (MAT) markers amplifying both idiomorphs were also developed to determine mating type distribution in populations.

Název v anglickém jazyce

Development of microsatellite and mating type markers for the pine needle pathogen Lecanosticta acicola

Popis výsledku anglicky

Lecanosticta acicola is an ascomycete that causes brown spot needle blight of pine species in many regions of the world. This pathogen is responsible for a major disease of Pinus palustris in the USA and is a quarantine organism in Europe. In order to study the genetic diversity and patterns of spread of L. acicola, eleven microsatellite markers and two mating type markers were developed. An enrichment protocol was used to isolate microsatellite-rich DNA regions, and 18 primer pairs were designed to flank these regions, of which eleven were polymorphic. A total of 93 alleles were obtained across all loci from forty isolates of L. acicola from the USA with an allelic diversity range of 0.095 to 0.931 per locus. Cross-species amplification with some of the markers was obtained with L. gloeospora, L. guatemalensis and Dothistroma septosporum, but not with D. pini. Mating type (MAT) markers amplifying both idiomorphs were also developed to determine mating type distribution in populations.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/EE2.3.20.0265" target="_blank" >EE2.3.20.0265: Indikátory vitality dřevin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Australasian Plant Pathology

ISSN

0815-3191

e-ISSN

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Svazek periodika

43

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

161-165

Kód UT WoS článku

332674600007

EID výsledku v databázi Scopus

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