Detection and quantification of airborne inoculum of Hymenoscyphus pseudoalbidus using real-time PCR assays

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F14%3A00228864" target="_blank" >RIV/62156489:43410/14:00228864 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/ppa.12218/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/ppa.12218/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/ppa.12218" target="_blank" >10.1111/ppa.12218</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection and quantification of airborne inoculum of Hymenoscyphus pseudoalbidus using real-time PCR assays

Popis výsledku v původním jazyce

A method based on real time PCR and using rotating arm spore traps was developed to quantify Hymenoscyphus pseudoalbidus airborne ascospores. The method was very sensitive and reproducible. The collection efficiency was evaluated to 10-15% of spores present in the air. The temporal ascospore dispersal pattern was studied over 3 years by collecting spores every 15 days during the ash growing season. The highest production was detected from the end of June to the beginning of September. The period of ascospore dispersal did not differ significantly among stands for a specific year. In contrast, there were differences according to the year. A positive correlation was calculated between the air temperature and the amount of ascospores trapped, with most ofthe positive samples observed above 12°C. The vertical profile of ascospore dispersal revealed a strong decrease in ascospore density within 3 m height regardless of the date of collection. Spore traps installed at increasing distances f

Název v anglickém jazyce

Detection and quantification of airborne inoculum of Hymenoscyphus pseudoalbidus using real-time PCR assays

Popis výsledku anglicky

A method based on real time PCR and using rotating arm spore traps was developed to quantify Hymenoscyphus pseudoalbidus airborne ascospores. The method was very sensitive and reproducible. The collection efficiency was evaluated to 10-15% of spores present in the air. The temporal ascospore dispersal pattern was studied over 3 years by collecting spores every 15 days during the ash growing season. The highest production was detected from the end of June to the beginning of September. The period of ascospore dispersal did not differ significantly among stands for a specific year. In contrast, there were differences according to the year. A positive correlation was calculated between the air temperature and the amount of ascospores trapped, with most ofthe positive samples observed above 12°C. The vertical profile of ascospore dispersal revealed a strong decrease in ascospore density within 3 m height regardless of the date of collection. Spore traps installed at increasing distances f

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GK - Lesnictví

OECD FORD obor

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Projekt

<a href="/cs/project/EE2.3.20.0265" target="_blank" >EE2.3.20.0265: Indikátory vitality dřevin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Pathology

ISSN

0032-0862

e-ISSN

—

Svazek periodika

63

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

1296-1305

Kód UT WoS článku

344914200009

EID výsledku v databázi Scopus

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