The Effects of Propagation Techniques on Leaf Vascular Anatomy, Modulus of Elasticity, and Photosynthetic Traits in Micropropagated and Grafted Plants of the Dutch Elm Hybrid 'Dodoens'

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F16%3A43910874" target="_blank" >RIV/62156489:43410/16:43910874 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The Effects of Propagation Techniques on Leaf Vascular Anatomy, Modulus of Elasticity, and Photosynthetic Traits in Micropropagated and Grafted Plants of the Dutch Elm Hybrid 'Dodoens'

Popis výsledku v původním jazyce

Understanding how plants are able to change their structural, physiological, and mechanical properties in response to various propagation methods can help to improve both their performance and their survival when transferred to field conditions. To identify changes between the routinely applied vegetative propagation techniques of in vitro micropropagation and splice grafting we assessed leaf performance for any differences in midrib vascular traits, nanomechanical properties of tracheary element cell walls, and photosynthetic traits in the dutch elm hybrid cultivar Dodoens (i.e., open-pollinated Ulmus glabra 'Exoniensis' x Ulmus wallichiana P39). In the micropropagated plants, the water-conducting area within the primary xylem tissue contained a significantly greater number of tracheary elements which suggests hydraulic safety. In the grafts, the water-conducting area contained a significantly smaller number of tracheary elements, in which the lumen areas were slightly larger than those of the micropropagated plants, resulting in a significantly higher size to number ratio which may indicate a fast and more effective water transport system. Quantitative nanomechanical mapping measurements from atomic force microscopy (AFM) revealed that the tracheary elements of the micropropagated plants formed stiffer cell walls quantified by the reduced Young's modulus of elasticity (MOE) than those of the grafts. The micropropagated plants were subjected to a more sensitive stomatal regulation of gas exchange resulting in the lower rates of net photosynthesis and transpiration. But the higher values of both instantaneous water-use efficiency (WUEinst) and chlorophyll a fluorescence yields found in the micropropagated plants indicate a higher acclimation capacity to stressful environmental conditions specifically for this stock type. Both stock types formed compact homogeneous clusters clearly separated from each other in the multivariate leaf trait analysis.

Název v anglickém jazyce

The Effects of Propagation Techniques on Leaf Vascular Anatomy, Modulus of Elasticity, and Photosynthetic Traits in Micropropagated and Grafted Plants of the Dutch Elm Hybrid 'Dodoens'

Popis výsledku anglicky

Understanding how plants are able to change their structural, physiological, and mechanical properties in response to various propagation methods can help to improve both their performance and their survival when transferred to field conditions. To identify changes between the routinely applied vegetative propagation techniques of in vitro micropropagation and splice grafting we assessed leaf performance for any differences in midrib vascular traits, nanomechanical properties of tracheary element cell walls, and photosynthetic traits in the dutch elm hybrid cultivar Dodoens (i.e., open-pollinated Ulmus glabra 'Exoniensis' x Ulmus wallichiana P39). In the micropropagated plants, the water-conducting area within the primary xylem tissue contained a significantly greater number of tracheary elements which suggests hydraulic safety. In the grafts, the water-conducting area contained a significantly smaller number of tracheary elements, in which the lumen areas were slightly larger than those of the micropropagated plants, resulting in a significantly higher size to number ratio which may indicate a fast and more effective water transport system. Quantitative nanomechanical mapping measurements from atomic force microscopy (AFM) revealed that the tracheary elements of the micropropagated plants formed stiffer cell walls quantified by the reduced Young's modulus of elasticity (MOE) than those of the grafts. The micropropagated plants were subjected to a more sensitive stomatal regulation of gas exchange resulting in the lower rates of net photosynthesis and transpiration. But the higher values of both instantaneous water-use efficiency (WUEinst) and chlorophyll a fluorescence yields found in the micropropagated plants indicate a higher acclimation capacity to stressful environmental conditions specifically for this stock type. Both stock types formed compact homogeneous clusters clearly separated from each other in the multivariate leaf trait analysis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GK - Lesnictví

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of the American Society for Horticultural Science

ISSN

0003-1062

e-ISSN

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Svazek periodika

141

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

351-362

Kód UT WoS článku

000385005000005

EID výsledku v databázi Scopus

2-s2.0-84982845673