Assessing the potential of lignin nanoparticles as drug carrier: Synthesis, cytotoxicity and genotoxicity studies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F20%3A43917657" target="_blank" >RIV/62156489:43410/20:43917657 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.ijbiomac.2020.02.311" target="_blank" >https://doi.org/10.1016/j.ijbiomac.2020.02.311</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2020.02.311" target="_blank" >10.1016/j.ijbiomac.2020.02.311</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Assessing the potential of lignin nanoparticles as drug carrier: Synthesis, cytotoxicity and genotoxicity studies

Popis výsledku v původním jazyce

Lignin nanoparticles synthesis is among recent developments in lignin valorization especially for biomedical applications. In this study, a new technique where complete self-assembling of lignin was ensured by simultaneous solvent displacement and flash pH change was used to optimize particle size of blank lignin nanoparticles (BLNPs) for suitability in cell uptake along with maximized yield. To establish BLNPs as drug carrier, safety studies including hemocompatibility, cytotoxicity and elaborate genotoxicity studies on Drosophila melanogaster as a model organism were done. Finally, irinotecan loaded lignin nanoparticles (DLNPs) were synthesized to establish their drug carrying potential and thorough in vitro characterization was performed. BLNPs with controllable size (⁓152 nm), low polydispersity (&lt;0.2), maximized yield (&gt;65%), negative surface charge (MINUS SIGN 22 to MINUS SIGN 23 mV), spherical shape and smooth surface were obtained with acceptable %hemolysis (&lt;2%). In vitro cytotoxicity studies revealed that BLNPs were significantly toxic (74.38 +- 4.74%) in human breast adenocarcinoma (MCF-7), slightly toxic (38.8 +- 4.70%) in human alveolar epithelial adenocarcinoma (A-549) and insignificantly toxic (15.89 +- 2.84%) to human embryonic kidney (HEK-293) cells. BLNPs showed concentration dependent early neuronal defects in Drosophila, but nuclei fragmentation and gut cell damage were absent. Sustained release DLNPs with high drug loading reduced the IC50 value of irinotecan by almost 3 folds.

Název v anglickém jazyce

Assessing the potential of lignin nanoparticles as drug carrier: Synthesis, cytotoxicity and genotoxicity studies

Popis výsledku anglicky

Lignin nanoparticles synthesis is among recent developments in lignin valorization especially for biomedical applications. In this study, a new technique where complete self-assembling of lignin was ensured by simultaneous solvent displacement and flash pH change was used to optimize particle size of blank lignin nanoparticles (BLNPs) for suitability in cell uptake along with maximized yield. To establish BLNPs as drug carrier, safety studies including hemocompatibility, cytotoxicity and elaborate genotoxicity studies on Drosophila melanogaster as a model organism were done. Finally, irinotecan loaded lignin nanoparticles (DLNPs) were synthesized to establish their drug carrying potential and thorough in vitro characterization was performed. BLNPs with controllable size (⁓152 nm), low polydispersity (&lt;0.2), maximized yield (&gt;65%), negative surface charge (MINUS SIGN 22 to MINUS SIGN 23 mV), spherical shape and smooth surface were obtained with acceptable %hemolysis (&lt;2%). In vitro cytotoxicity studies revealed that BLNPs were significantly toxic (74.38 +- 4.74%) in human breast adenocarcinoma (MCF-7), slightly toxic (38.8 +- 4.70%) in human alveolar epithelial adenocarcinoma (A-549) and insignificantly toxic (15.89 +- 2.84%) to human embryonic kidney (HEK-293) cells. BLNPs showed concentration dependent early neuronal defects in Drosophila, but nuclei fragmentation and gut cell damage were absent. Sustained release DLNPs with high drug loading reduced the IC50 value of irinotecan by almost 3 folds.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Biological Macromolecules

ISSN

0141-8130

e-ISSN

—

Svazek periodika

152

Číslo periodika v rámci svazku

1 June

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

17

Strana od-do

786-802

Kód UT WoS článku

000530068000078

EID výsledku v databázi Scopus

2-s2.0-85081321551