Survey and Monitoring of Phytophthora Species in Natural Ecosystems: Methods for Sampling, Isolation, Purification, Storage, and Pathogenicity Tests

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F22%3A43921792" target="_blank" >RIV/62156489:43410/22:43921792 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/978-1-0716-2517-0_2" target="_blank" >https://doi.org/10.1007/978-1-0716-2517-0_2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-0716-2517-0_2" target="_blank" >10.1007/978-1-0716-2517-0_2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Survey and Monitoring of Phytophthora Species in Natural Ecosystems: Methods for Sampling, Isolation, Purification, Storage, and Pathogenicity Tests

Popis výsledku v původním jazyce

Phytophthora species can be found in multiple substrates. Due to dormancy of resting structures and presence of faster-growing antagonists, direct isolation of Phytophthora can be difficult to achieve, and indirect baiting methods often reach higher isolation frequencies. In this chapter, different methodologies are described for sampling and for the successful isolation of Phytophthora species from natural ecosystems. Sampling methods for soil, roots, bark cankers, and waterbodies are described. Agar recipes and guidance on the selection of suitable tissue to perform direct isolations are provided. A range of different baiting techniques are described for isolation of Phytophthora from different substrates. Purification methods to obtain clean and non-mixed cultures and conservation methods for pure cultures are shown. Two pathogenicity test methods for soil infestation and for under-bark inoculation, respectively, are described in detail.

Název v anglickém jazyce

Survey and Monitoring of Phytophthora Species in Natural Ecosystems: Methods for Sampling, Isolation, Purification, Storage, and Pathogenicity Tests

Popis výsledku anglicky

Phytophthora species can be found in multiple substrates. Due to dormancy of resting structures and presence of faster-growing antagonists, direct isolation of Phytophthora can be difficult to achieve, and indirect baiting methods often reach higher isolation frequencies. In this chapter, different methodologies are described for sampling and for the successful isolation of Phytophthora species from natural ecosystems. Sampling methods for soil, roots, bark cankers, and waterbodies are described. Agar recipes and guidance on the selection of suitable tissue to perform direct isolations are provided. A range of different baiting techniques are described for isolation of Phytophthora from different substrates. Purification methods to obtain clean and non-mixed cultures and conservation methods for pure cultures are shown. Two pathogenicity test methods for soil infestation and for under-bark inoculation, respectively, are described in detail.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

40102 - Forestry

Projekt

<a href="/cs/project/EF15_003%2F0000453" target="_blank" >EF15_003/0000453: Výzkumné centrum pro studium patogenů z rodu Phytophthora</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Plant Pathology: Methods and Protocols

ISBN

978-1-07-162516-3

Počet stran výsledku

37

Strana od-do

13-49

Počet stran knihy

497

Název nakladatele

Humana Press Inc./Springer

Místo vydání

Totowa

Kód UT WoS kapitoly

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