What does critical temperature tell us about the resistence of polar lichens to freezing stress? Applicability of linear cooling method to ecophysiological studies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F22%3A43923195" target="_blank" >RIV/62156489:43410/22:43923195 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/22:00128890

Výsledek na webu

<a href="https://dx.doi.org/10.5817/CPR2022-2-18" target="_blank" >https://dx.doi.org/10.5817/CPR2022-2-18</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5817/CPR2022-2-18" target="_blank" >10.5817/CPR2022-2-18</a>

Jazyk výsledku

angličtina

Název v původním jazyce

What does critical temperature tell us about the resistence of polar lichens to freezing stress? Applicability of linear cooling method to ecophysiological studies

Popis výsledku v původním jazyce

Lichens from polar regions are well adapted to low temeprature and considered cryoresistant. However, interspecific differences in their cryoresistence exist according to the degree of their adaptation and severity of the environment. In our study, we applied linear cooling technique in order to evaluate the interspecific differences in several lichen species. Thalli segments of Umbilicaria antarctica, Nephroma antarctica, Placopsis contortuplicata and Lasallia pustulata were exposed to the cooling from 20 to -35 degrees C at a constant rate of 2 degrees C min(-1). Simultaneously with the cooling, chlorophyll fluorescence parameters evaluating potential (F-V/F-M) and effective yield of primary photochemical processes in PSII (Phi(PSII)) were measured in 30 s interval. Temperature re-sponse curves of F-V/F-M and Phi(PSII) formed typical S-curves that were species specific. Critical temperature (cooling point at which Phi(PSII) equals 0), was found in a narrow range of -25 to -28 degrees C, suggesting that all experimental lichen species have a high resistance to sub-zero temperatures. The method of linear cooling used in this study has proven its applicability in ecophysiological studies since it is sensitive enough for the evaluation of species-specific differences in cryoresistence. This study describes different parameters that can be derived from the S-curves and discuss their proper use in ecophysiological and stress physiology studies.

Název v anglickém jazyce

What does critical temperature tell us about the resistence of polar lichens to freezing stress? Applicability of linear cooling method to ecophysiological studies

Popis výsledku anglicky

Lichens from polar regions are well adapted to low temeprature and considered cryoresistant. However, interspecific differences in their cryoresistence exist according to the degree of their adaptation and severity of the environment. In our study, we applied linear cooling technique in order to evaluate the interspecific differences in several lichen species. Thalli segments of Umbilicaria antarctica, Nephroma antarctica, Placopsis contortuplicata and Lasallia pustulata were exposed to the cooling from 20 to -35 degrees C at a constant rate of 2 degrees C min(-1). Simultaneously with the cooling, chlorophyll fluorescence parameters evaluating potential (F-V/F-M) and effective yield of primary photochemical processes in PSII (Phi(PSII)) were measured in 30 s interval. Temperature re-sponse curves of F-V/F-M and Phi(PSII) formed typical S-curves that were species specific. Critical temperature (cooling point at which Phi(PSII) equals 0), was found in a narrow range of -25 to -28 degrees C, suggesting that all experimental lichen species have a high resistance to sub-zero temperatures. The method of linear cooling used in this study has proven its applicability in ecophysiological studies since it is sensitive enough for the evaluation of species-specific differences in cryoresistence. This study describes different parameters that can be derived from the S-curves and discuss their proper use in ecophysiological and stress physiology studies.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/EF16_013%2F0001708" target="_blank" >EF16_013/0001708: ECOPOLARIS - Změny ve struktuře a funkci součástí terestrických polárních ekosystémů (CzechPolar2)</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Polar Reports

ISSN

1805-0689

e-ISSN

1805-0697

Svazek periodika

12

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

246-255

Kód UT WoS článku

000935360100008

EID výsledku v databázi Scopus

2-s2.0-85151484685