Analýza genetické diverzity v rámci vybraných genotypů rodu Prunus pomocí metod RAPD a SSR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F06%3A00092407" target="_blank" >RIV/62156489:43510/06:00092407 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparative analysis of genetic diversity in Prunus L. as revealed by RAPD and SSR markers

Popis výsledku v původním jazyce

RAPD and SSR markers were used for genetic diversity evaluations among 15 genotypes selected from the genus Prunus L. Altogether 40 RAPD primers and 21 primer pairs designated for microsatellite loci were applied on the whole group of genotypes. The RAPDprimers and SSR loci with the highest degree of observed polymorphism were selected. Three approaches were used to generate DNA-based genetic markers--RAPD, SSR with separations on PAGE, SSR with separations on MetaPhor agarose. An analysis of each approach was statistically summarized and the obtained parameters were compared. On the basis of the data obtained by individual methods, the relevant dendrograms of genetic similarities among genotypes were constructed. The Mantel tests between individual similarity matrices showed a statistically significant correlation between the RAPD- and SSR-based similarities if computed for the whole group of analyzed genotypes. Correlations hugely decrease in the case of a parallel analysis of a low

Název v anglickém jazyce

Comparative analysis of genetic diversity in Prunus L. as revealed by RAPD and SSR markers

Popis výsledku anglicky

RAPD and SSR markers were used for genetic diversity evaluations among 15 genotypes selected from the genus Prunus L. Altogether 40 RAPD primers and 21 primer pairs designated for microsatellite loci were applied on the whole group of genotypes. The RAPDprimers and SSR loci with the highest degree of observed polymorphism were selected. Three approaches were used to generate DNA-based genetic markers--RAPD, SSR with separations on PAGE, SSR with separations on MetaPhor agarose. An analysis of each approach was statistically summarized and the obtained parameters were compared. On the basis of the data obtained by individual methods, the relevant dendrograms of genetic similarities among genotypes were constructed. The Mantel tests between individual similarity matrices showed a statistically significant correlation between the RAPD- and SSR-based similarities if computed for the whole group of analyzed genotypes. Correlations hugely decrease in the case of a parallel analysis of a low

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientia Horticulturae

ISSN

0304-4238

e-ISSN

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Svazek periodika

108

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

253-259

Kód UT WoS článku

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EID výsledku v databázi Scopus

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